Prevention of photoaging by scavenging mitochondrial singlet oxygen

由于人们渴望看起来年轻美丽，延缓衰老的化妆品是一个很大的市场。皱纹的形成归因于UVA辐射。利用在表皮角质细胞细胞质和线粒体中表达氧化还原敏感GFP （roGFP1）的无毛转基因小鼠，我们发现UVA诱导皮肤线粒体氧化应激，而不是细胞质。我们使用体内氧化应激成像来评估单线态氧清除剂。局部应用类胡萝卜素不能预防uva诱导的氧化应激。我们认为这是由于大的类胡萝卜素穿过角质层的渗透性差。然而，相对于载体控制，能够很好地渗透角质层的低分子量化合物可以减少UVA诱导的氧化量。未来的研究将进一步研究可以通过局部应用于皮肤来防止UVA或可见光引起的氧化应激的物质。

Due to a high desire to look young and beautiful, cosmetics to slow down aging are a large market. Wrinkle formation has been attributed to UVA radiation.Using hairless transgenic mice expressing a redox-sensitive GFP (roGFP1) in the cytosol and in mitochondria of epidermal keratinocytese, we discovered that UVA induces oxidative stress in skin mitochondria but not cytosol. We used in vivo oxidative stress imaging to evaluate singlet oxygen scavengers. Topical application of carotenoids did not prevent UVA-induced oxidative stress. We assume this was due to poor permeation of the large carotenoids across the stratum corneum. However, a low molecular weight compound able to permeate the stratum corneum well could reduce the amount of oxidation induced by UVA relative to vehicle control.Future research will further investigate substances that can prevent UVA or visible light-induced oxidative stress by topical application to skin.