Development of a translation system using expanded genetic alphabets through unnatural base pairs

为了扩展DNA的遗传字母表，我们研究了几个密码子-反密码子在无细胞翻译系统中的相互作用，包括疏水性的非天然碱基。在本项目中，我们主要致力于建立高效的转录系统及其模板DNA制备方法，这些方法允许将疏水性非天然碱基以所需的量掺入DNA和RNA中。从这个项目中发现的结果将打开一个翻译系统的进一步发展，涉及非天然碱基对正交的天然碱基对。

For the expansion of the genetic alphabet of DNA, we studied several codon-anticodon interactions in a cell-free translation system involving hydrophobic unnatural bases. For this project, we mainly worked on to establish efficient transcription systems and their template DNA preparation methods which allow incorporation of hydrophobic unnatural bases at desired potions into DNA and RNA. The results found from this project would open up further development on a translation system involving unnatural base pairs orthogonal to natural base pairs.