NMR studies of the structure of precipitated calcium phosphates and carbonates and minral-protein interactions

了解生物矿化过程的进展需要在此过程中形成的各种物种的详细和定量的结构信息。无序相在生物矿化、矿物晶体的初始成核、纳米晶体表面以及最重要的蛋白质-矿物相互作用中具有特别重要的作用。本提案的目的是使用核磁共振（具有特殊和重要的优势，即它不会受到样品中无序或矿物晶体纳米尺寸的限制）来详细研究各种蛋白质（骨钙素，骨桥蛋白，fetuin， o-磷酸- l-丝氨酸）存在下沉淀磷酸钙的结构和矿物-蛋白质相互作用。另一个主要目的是详细研究非晶碳酸钙（ACC）的结构，它是x射线非晶的，它的老化（热和水中）和再结晶过程的动力学。

Progress in understanding biomineralization processes requires detailed and quantitative structural information on the various species being formed in the process. Disordered phases are of particular importance in biomineralization, in the initial nucleation of the mineral crystals, on the surface of the nanocrystals and -most importantly- in the protein-mineral interaction. The aim of this proposal is to use NMR (with the specific and important advantage that it does not suffer from limitations due to disorder in the sample or the nanoscopic dimensions of the mineral crystals) to study in detail the structure of precipitated calcium phosphates in the presence of various proteins (osteocalcin, osteopontin, fetuin, O-phospo-L-serine) and the mineral-protein interaction. Another principal objective is the detailed investigation of the structure of amorphous calcium carbonate (ACC) which is X-ray amorphous, its ageing (thermal and in water) and the kinetics of the recrystallization processes.