Development of component resolved diagnostics to predict the clinical relevance and time course of food allergy in childhood

对食物的过敏反应在生命的最初几年影响了多达8%的儿童。这些儿童中的大多数在以后的生活中会获得耐受性，尽管20-30%的儿童在成年后仍会保持过敏。此外，食物过敏通常先于以后生活中过敏性气道疾病的发展。到目前为止，没有单一的实验室参数证明食物过敏的诊断或疾病的自然时间进程。在当前的提案中，将建立用于诊断食物过敏及其自然时间过程的预测的组分分辨诊断。我们的目标是使用微阵列技术开发“食物过敏原芯片”。将使用已知的牛奶、鸡蛋和花生的纯化或重组变应原蛋白以及代表已知IgE结合位点的肽。过敏原和肽特异性IgE-和IgG 4-抗体的存在和变化将与食物过敏症状的临床和持续性以及呼吸道过敏的发展相关。将使用来自三个独立队列的儿童血清，并对其进行纵向跟踪。此外，我们将使用蛋白质组学方法来鉴定和表征已知的临床相关过敏性小麦蛋白。该项目将是开发工具的又一步，使我们能够预测未来治疗干预的特应性疾病的发展。

Allergic reactions to food affect up to 8% of children in the first years of life. Most of these children will gain tolerance later in life, although 20-30% retains their allergy until adulthood. Moreover, food allergy precedes often the development of allergic airway diseases later in life. Up to date, no single laboratory parameter proofs the diagnosis of food allergy or the natural time course of the disease. In the current proposal, component resolved diagnostics for the diagnostic of food allergy and the prediction of its natural time course will be established. Our goal is the development of a “food allergen chip” using a microarray technology. Known purified or recombinant allergenic proteins of cow’s milk, hen’s egg and peanut as well as peptides representing the known IgE-binding sites will be used. The presence and changes of allergen and peptide-specific IgE- and IgG4-antibodies will be correlated with the clinic and persistence of food allergic symptoms as well as with the development of respiratory allergies. Sera of children from three independent cohorts that were followed longitudinal will be used. Moreover, we will use proteomic approaches to identify and characterize knew clinical relevant allergenic wheat proteins. This project will be another step in the development of tools that enable us to predict the development of atopic diseases for future therapeutic interventions.