AFM-based determination of interface and interphase interactions

在目前的资助期内，我们与Regine v. Klitzing小组合作，使用原子力显微镜（AFM）来表征聚电解质多层膜（PEMs）的厚度和膨胀行为。此外，我们建立了由共价连接到afm尖端的单个分子组成的分子探针，并获得了垂直于表面（即间相）的PEMs粘附能谱的第一个数据。在第二个资助期，我们将使用我们的基于afm的分子探针来完成PEMs中的粘附能和含水量谱。主要重点将是利用我们基于原子力显微镜的方法确定多层材料的横向和垂直流动性。这些测量将由我实验室的单分子荧光测量和Regine v. Klitzing小组的散射实验来补充。特别令人感兴趣的是分子量依赖性、电荷依赖性、固体载体和离子特异性效应的影响。然后，这些研究将应用于SPP中研究的其他（未带电的）聚合物界面，即聚苯乙烯和聚丁二烯基聚合物。最后，由于系统尺寸小，结果可以直接与Christian Holm在同一系统上进行的md模拟进行比较。

During the current funding period we used atomic force microscopy (AFM) to characterize the thickness and swelling behaviour of polyelectrolyte multilayers (PEMs) in collaboration with the group of Regine v. Klitzing. In addition, we established molecular probes consisting of a single molecule covalently attached to an AFM-tip and got first data on the adhesion energy profile of PEMs perpendicular to the surface (i.e. in the interphase). In the second funding period we will use our AFM-based molecular probes to complete adhesion energy and water content profiles in PEMs. The main focus will be on determining the lateral and perpendicular mobility throughout the multilayer with our AFM-based method. These measurements will be complemented by single molecule fluorescence measurements in my lab and scattering experiments in the group of Regine v. Klitzing. Of particular interest are the molecular weight dependence, charge dependence, the influence of the solid support and ions specific effects. These investigations will then be applied to other (uncharged) polymer interphases investigated in the SPP, namely polystyrene and polybutadiene based polymers. Finally, because of the small system size, the results can directly be compared to MD-simulations performed on the same system by Christian Holm.