Deposition Mechanism of Inorganic Material in Denture Plaque

1.义齿菌斑和结石的观察用透射电镜和电子探针研究了义齿菌斑和结石的形成过程，结果表明，在3d时，义齿表面松散结合的微生物间隙中出现了无定形的电子致密物质，而在6 h时则未观察到菌斑。患者义齿表面出现层状物质（LS），且矿化严重。这些结果表明，牙菌斑促进了钙的积累，牙结石的形成是由微生物间基质、细胞膜和/或LS启动的.原子力显微镜（SPM-9500）在义齿表面观察中的应用新近引进的原子力显微镜能使我们更清楚地三维观察义齿表面的微小结构，如粗糙度，它配备了图像处理计算机。通过原子力显微镜与传统扫描电镜对义齿表面粗糙度的对比观察，评价了原子力显微镜的观察效果，原子力显微镜能更准确地获得义齿表面粗糙度的图像。因此，原子力显微镜是研究观察义齿表面沉积的牙菌膜、牙菌斑和牙结石等受力情况的有效设备。

1. Observation of denture plaque and denture calculusThe formation process of the denture plaque and calculus were studied by means of transmission electron microscopy and EPMA.Amorphous electron dense materials appeared in the spaces between the loosely bound microorganisms on the denture surface at 3days, although the denture plaque was not observed at 6 hours. The lamellated substance (LS) appeared on the surface of the denture from the patients, and the substance was heavily mineralized. The initial mineral crystals appeared along the plasma membrane of microorganisms, the intermicrobiol matrix, and the LS.These results suggested that the denture plaque facilitates the calcium accumulation and the formation of the denture calculus is initiated by the interminicrobiol matrix, the cell membrane, and/or the LS.2. Application of Atomic Force Microscope(SPM-9500) to observation of denture surfaceThe recently introduced atomic force microscope can enable us to three-dimensionally observe the minute surface structures of the denture like ruggedness more clearly, being equipped with an image processing computer. Its observation efficacy was evaluated through comparative observations of denture surfaces between an atomic force microscope and a traditional scanning electron microscope.More accurate images of surface ruggedness were more easily obtained by employing the newly introduced atomic force microscope. Therefore , it was concluded that the atomic force microscope is an effective device for research observation of the denture surface deposited with pellicle, denture plaque and denture calculus and so force.