Cariogenicity of Denture Plaque

假牙牙菌斑的致龋性

• 批准号: 11671934
• 负责人: NISHIMURA Masahiro
• 金额: $ 2.3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671934/
• 关键词: Candida albicans; collagenase; Root caries; collagen; Actinomyces; Lactobacillus; cariogenicity; Streptococcus mutans; Condida albicans; コラーゲン; コラゲナーゼ

It is noteworthy that the cariogenicity depends not only on the terminal pH, but also on several other factors, including the ability of the organism to adhere or colonize the tooth surface. At this project, we found that the adhesion of C.albicans to intact and denatured type I collagen was significantly greater than those of oral bacteria and other species of Candida.Also we found that C.albicans degrade intact collagen sponge when C.albicans cultured in the presence of bovine serum albumin in culture medium.Cell associated collagenolytic activity of C.albicans was quantitated by measuring the degradation of synthetic collagenolytic peptide 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA), which is a specific substrate for collagenase. Cell associated collagenolytic activity of Candida albicans was relatively lower than that produced by Streptococcus mutans or Actinomyces. Although it was almost same level as that of lactobacillus.. This collagenolytic activity was enhanced by the cells cultured in the presence of bovine serum albumin in culture medium and this activity was inhibited in the presence of EDTA, but not by the serine proteinase inhibitor (APMSF) and the aspartyl proteinase inhibitor (Pepstatin A).Analyze of conditioned medium of C.albicans by gelatin zymography shows 116k and 150kDa bands. These bands were disappear by boiling of the sample, though no effect by the treatment with EDTA.These results indicate that C.albicans produces several collagenolytic enzymes and which may be a factor involved in pathogenesis of root caries.

值得注意的是，致龋性不仅取决于终末pH，还取决于其他几个因素，包括生物体粘附或定殖牙齿表面的能力。在这个项目中，结果表明，白念珠菌对完整的和变性的I型胶原的粘附能力显著高于口腔细菌和其他念珠菌，同时发现白念珠菌在牛血清白蛋白存在的培养基中培养时，可降解完整的胶原海绵，通过测定合成的胶原溶解肽的降解，定量分析白念珠菌的细胞相关胶原溶解活性2-呋喃丙烯酰基-Leu-Gly-Pro-Ala（GlyGPA），其是胶原酶的特异性底物。白念珠菌的细胞相关胶原溶解活性相对低于变形链球菌和放线菌。虽然它几乎是相同的水平，作为一个..白念珠菌条件培养液经明胶酶谱分析显示116 k和150 kDa条带，丝氨酸蛋白酶抑制剂（APMSF）和胰蛋白酶抑制剂（Pepstatin A）对白念珠菌的胶原蛋白溶解活性无明显影响。结果表明，白色念珠菌能产生多种胶原酶，这些酶可能是根龋发病的一个因素。

项目成果

Makihira, S., et al.: "Bacterial and candidal adhesion to intact and denatured collagen in vitro."Mycoses. (in press).

Makihira, S. 等人：“细菌和念珠菌在体外粘附于完整和变性的胶原蛋白。”真菌病。

山城啓文: "Candida albicansと根面齲蝕の関連性に関する研究"広島大学歯学会雑誌. 32・1. 1-16 (2000)

Hirofumi Yamashiro：“白色念珠菌与根龋的关系的研究”广岛大学牙科学会杂志32・1（2000）。

Makihira,S. et al: "Bacterial and candidal adhesion to intact and denatured collagen in vitro."Mycoses. (印刷中).

Makihira, S. 等人：“细菌和念珠菌在体外粘附于完整和变性的胶原蛋白。”真菌病（正在出版）。

Yamashiro, H.: "Possible role of Candida albicans in the decay of root surfaces."The J.Hiroshima Univ.Dent.Soc.. 32-1. 1-16 (2000)

Yamashiro, H.：“白色念珠菌在根表面腐烂中的可能作用。”J.Hiroshima Univ.Dent.Soc. 32-1。

山城啓文: "Candida albicansと根面齲蝕の関連性に関する研究"広島大学歯学会雑誌. 32. 1-16 (2000)

Hirofumi Yamashiro：“白色念珠菌与根龋的关系研究”广岛大学牙科学会杂志 32. 1-16 (2000)。