Basis Study for Production of Artificial Nerve System to Use for Peripheral Nerve Defects

因头颈部疾病导致畸形的患者。这些畸形是通过重建手术来治疗的。自体骨、肌肉、皮肤、神经等是可靠的生物材料。实际上，有用的生物材料的开发已经取得了进展，并且有许多生物材料可用于临床。此时，我计划进行生产用于周围神经缺损的人工神经系统的基础研究。首先，从大鼠坐骨神经中分离培养雪旺细胞，并在冻融处理后的雪旺细胞片上培养大鼠三叉神经节。结果以成纤维细胞培养为对照，采用组织学和免疫组化方法观察到三叉神经节在雪旺细胞培养上生长，提示雪旺细胞对神经再生有影响。这对制作用于周围神经缺损的人工神经系统是有益的。

Patients who have a deformity caused with head and neck diseases. These deformities are treated with reconstruction surgey. Autogenous bone, muscle, skin and nerve are used as reliable biomaterials. In fact, the developments of useful biomaterials have been progress and these are many biomaterials for clinic.At this time, I projected the research for basic study for production of artificial nerve system to use for peripheral nerve defects. First of all, Schwann cells that come from rats' sciatic nerve were cultured and rats' trigeminal ganglions were cultured on freeze-melt treatment Schwann cells' sheets. In the results with the cultured on fibroblast cells' sheets as a controls, trigeminal ganglions were lived on Schwann cells' sheets with the methods of histological and immunhistochemical technique.These results suggested that Schwann cells have an influence for nerve regeneration. It is useful for production of artificial nerve system to use for peripheral nerve defects.