A novel in vivo overexpression screen for cell cycle and growth regulating genes in Drosophila

为了鉴定体内调节细胞生长、细胞周期进程和增殖的基因，我打算在果蝇果蝇中进行一种新的过表达筛选。我将创建一个包含大约650个果蝇品系的文库，每条品系包含一个插入果蝇基因组特定位点的开放阅读框（ORF）。在基于RNAi的果蝇S2细胞功能丧失筛选中，选定的orf在细胞周期和细胞大小调节以及各种相关机制中发挥作用。然而，这些基因在多细胞生物体内的作用尚不清楚。我将筛选蝇系的各种生长相关表型。许多使用新技术和已经建立的技术的检测将识别与器官大小、细胞周期进展和细胞生长有关的基因。这项工作代表了对一组可能在大小调节和生长控制中发挥作用的基因的全面和系统的分析。此外，它是旨在建立全基因组过表达文库的更大项目的一部分。试点图书馆的经验对这个项目至关重要。

To identify genes that regulate cell growth, cell cycle progression and proliferation in vivo, I intend to perform a novel overexpression screen in the fruit fly Drosophila melanogaster. I will create a library of about 650 fly lines, each line containing one open reading frame (ORF) inserted into a specific locus of the Drosophila genome. The selected ORFs were identified in an RNAi based loss-of-function screen in Drosophila S2 cells to function in cell cycle and cell size regulation, as well as a variety of related mechanisms. However, the in vivo role of these genes in the context of a multicellular organism remains unclear. I will screen the fly lines for a variety of growth related phenotypes. A number of assays using new, as well as already established techniques will identify genes involved in organ size, cell cycle progression and cellular growth. This work represents a comprehensive and systematic analysis of a large set of genes that may play a role in size regulation and growth control. Furthermore, it is part of a larger project aimed at the establishment of a genome-wide overexpression library. Experiences from the pilot-library will be essential to this project.