Molecular Biologic Study on Etiological Mechanisms and Therapy of Glaucoma

1)免疫组织化学方法检测MYOC/TIGR蛋白在青光眼和正常眼小梁中的定位。光镜下所有标本的小梁网均呈抗MYOC/TIGR多克隆抗体阳性。细胞外基质染色观察。电镜免疫组织化学染色不仅见于小梁细胞的胞浆，还见于细胞外基质。在细胞外基质中，染色与长间隔的胶原和细小颗粒物质有关。结论：MYOC/TIGR蛋白不仅存在于小梁细胞中，而且还存在于细胞外基质中，与长间隔胶原和细小颗粒物质有关。2)对剥脱综合征患者眼球摘除后的小梁组织进行组织学和免疫组织化学检测。剥离纤维被发现含有蛋白多糖和一些…更多的其他大分子。3)对遗传性青光眼兔眼的角区进行了形态学观察。在青光眼的角区，在神经丛的正下方有一个厚厚的异常组织，细胞外基质中嵌入了圆形的细胞。厚壁组织中可见大量基板样物质的细胞外基质。在正常眼，角区由发达的小梁片组成。这些发现支持这一假设，即由于虹膜角膜角度发育不良而残留的厚厚的小管下组织是这种类型青光眼的主要原因之一。4)我们检测了新生血管性青光眼的人眼以及实验性眼前段新生血管的兔眼的小梁网。结果提示，生长因子(VEGF)在眼前段发育中起重要作用，新生小梁间隙的侵袭与青光眼的临床表现密切相关。5)免疫组织化学方法研究细胞外基质在正常眼组织中的定位。结果表明，层粘连蛋白和VI型胶原广泛分布于小梁网中。6)我们检查了左眼虹膜痣综合征患者小梁切除后的小梁组织，发现虹膜痣综合征患者的血-房水屏障可能发生破坏。7)我们用激光多普勒血流仪检测了富马酸布罗卡明和马来酸噻吗洛尔对脉络膜血流的影响。提示静脉给药可能不会增加脉络膜血流量。较少

1) We immunohistochemically examined the localization of MYOC/TIGR protein in the glaucomatous and normal trabecular meshworks. In light microscopic immunohistochemistry, the trabecular meshworks from all the specimens stained positively with anti-MYOC/TIGR polyclonal antibody. Staining was observed in the extracellular matrix. In electron microscopic immunohistochemistry, staining was seen not only in the cytoplasm of the trabecular cells but also in the extracellular matrix. In the extracellular matrix, staining was associated with the long-spacing collagens and fine granular materials. It is concluded that MYOC/TIGR protein is distributed not only in the trabecular cells but also in the extracellular matrix associating with the long-spacing collagens and fine granular materials.2) The trabecular tissues from enucleated human eyes with exfoliation syndrome were examined histologically and immunohistochemically. Exfoliation fibers were revealed to contain proteoglycans as well as some … More other macromolecules. It was also detected that the iris vessels had some abnormal changes in the syndrome.3) We examined morphologically the angular region of eyes with inherited glaucoma in rabbits. In the angular region of glaucomatous eyes, a thick abnormal tissue with round-formed cells embedded in the extracellular matrix was located just beneath the plexus. A large amount of extracellular matrix of basal lamina-like material was observed in the thick tissue. In normal eyes, the angular region consisted of well-developed trabecular sheets. These findings support the hypothesis that remaining of a thick subcanalicular tissue because of maldevelopment of the iridocorneal angle is one of the main causes of this type of glaucoma.4) We examined the trabecular meshwork from human eyes with neovascular glaucoma, as well as from rabbit eyes with experimental neovascularization in the anterior segment of the eye histologically and immunohistochemically. The results suggested growth factor (VEGF) plays an important role in development of anterior segment of the eye, and that invasion of the newly intertrabecular spaces has close relation to glaucoma manifestation.5) We studied the localization of extracellular matrix in the normal immunohistochemically. The results indicate that laminin and type VI collagen are located diffusely in the trabecular meshwork.6) We examined the trabecular tissue obtained by trabeculectomy from the patient's with iris nevus syndrome in the left eye to show that disruption of the blood-aqueous barrier may occur in iris-nevus syndrome.7) We examined the effects of brovincamine fumarate and timolol maleate on choroidal blood flow using laser Doppler flowmetry. The results indicated that intravenous administration of the drugs might not increase the choroidal blood flow. Less