MOLECULAR BIOLOGIC STUDY ON CORTICOSTEROID-INDUCED GLAUCOMA

皮质类固醇诱发青光眼的分子生物学研究

• 批准号: 07671925
• 负责人: TAWARA Akihiko
• 金额: $ 1.47万
• 依托单位: WAKAYAMA MEDICAL COLLEGE (1996)Kyushu University (1995)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671925/
• 关键词: corticosteroid-induced glaucoma; triamcinolone acetonide; histochemistry; extracellular matrix; Proteoglycans; exfoliation syndrome; goniodysgenetic glaucoma; neovascular glaucoma; ステロイド縁内障; 隅角発育異常縁内障; 血管新生縁内障; 接着分子; 免疫組織化学

We performed subtenon injection of 20 or 40 mg triamcinolone acetonide into right eyes of Japanese rabbits, and measured their intraocular pressures periodically for 4 weeks. The anterior chamber angle tissues obtained from the animals were examined histologically and histochemically using alcian blue or cupromeronic Blue. There was no significant increase of the intraocular pressure compared to control left eyes. No histological or histochemical change was detected in the trabecular tissues from corticosteroid injected eyes. The results suggest that there may be some difference in reactivity to corticosteroid between human and rabbit eyes.The effects of anterior segment ischemia on angiogenesis in the anterior segment of the eye were investigated. We cauterized both of the long posterior ciliary arteries in the right eye of 14 rabbits. The eyes were enucleated 1,2,4,7,9 and 14 days after cauterization and were studied with light microscopy. The localization of vascular endothelial gro … More wth factor (VEGF) protein and the expression of VEGF mRNA in the tissues were studied. Neovascularization on the anterior surface of the iris and in the trabecular meshwork was detected. No histological changes were observed in the retina. VEGF protein was detected in the epithelial and vascular cells of the iris. Expression of VEGF mRNA was detected in the epithelial cells of the ciliary body. These results suggest that anterior segment ischemia causes neovascularization in the anterior segment of the eye.The trabecular tissues from 4 enucleated human eyes with exfoliation syndrome were examined histologically and immunohistochemically. Exfoliation fibers were revealed to contain chondroitin sulfate dermatan sulfate and heparan sulfate-type proteoglycans as well as HNK-I epitope, while microfibrils in the extracellular matrix did not. The results suggest that depositions of proteoglycans and other macromolecules on the microfibrils may be closely associated with the formation of exfoliation materials.We examined morphologically the ratio of the ciliary body band width to the width of the posterior limbus, termed the theoretical C/L ratio, of enucleated human eyes, including those from premature infants as well as from older subjects. We also examined clinically the appearance of the ciliary body band on gonioscopy in patients having glaucoma with open iridocorneal angles, and in age mathched controls. The theoretical C/L ratio was 1.4(]SY.+-。[)2.7% in premature infants, 24.0(]SY.+-。[)5.4% in children and 36.7(]SY.+-。[)8.0% in the older subjects. On gonioscopy, 77.8% glaucomatous eyes and 13.8% control eyes showed either an invisible or very narrow ciliary body band. The results indicate that an invisible or very narrow ciliary body band represents an nuderdevelopment of the angle. Less

在日本兔右眼内注射曲安奈德20、40 mg，定期测量其眼压，持续4周。采用阿利新蓝或铜二甲基蓝对动物前房角组织进行组织学和组织化学检查。与对照组相比，眼压无明显升高。注射皮质类固醇后的眼小梁组织未见组织学或组织化学变化。结果表明，人与兔眼睛对皮质类固醇的反应性可能存在一定差异。研究了眼前段缺血对眼前段血管生成的影响。我们烧灼了14只兔右眼的两条纤毛后长动脉。灼烧后1、2、4、7、9、14天分别去核，光镜下观察。研究了血管内皮生长因子（VEGF）蛋白在组织中的定位及VEGF mRNA的表达。在虹膜前表面和小梁网中发现了新生血管。视网膜未见组织学改变。在虹膜上皮细胞和血管细胞中检测到VEGF蛋白。在纤毛体上皮细胞中检测到VEGF mRNA的表达。这些结果表明，前段缺血引起眼前段的新生血管形成。对4例去核脱落综合征患者的眼小梁组织进行了组织学和免疫组织化学检查。脱落纤维含有硫酸软骨素、硫酸皮肤素和硫酸肝素型蛋白多糖以及HNK-I表位，而细胞外基质中的微原纤维则不含。结果表明，蛋白聚糖和其他大分子在微原纤维上的沉积可能与脱落物质的形成密切相关。我们从形态学上检查了包括早产儿和老年受试者在内的去核人眼睫状体带宽度与后缘宽度的比值，称为理论C/L比值。我们还在临床上检查了虹膜角膜角开放的青光眼患者和年龄匹配的对照组的睫状体带的外观。理论C/L比值早产儿为1.4(]SY.+-[)2.7%，儿童为24.0(]SY.+-[)5.4%，老年受试者为36.7(]SY.+-[)8.0%。在角膜镜检查中，77.8%的青光眼和13.8%的对照眼显示睫状体带不可见或很窄。结果表明，一个不可见或很窄的纤毛体带代表了角的裸发育。少

项目成果

Toshiaki Kubota, Akihiko Tawara, et al.: "Neovascular tissue in the intertrabecular spaces in eyes with neovascular glaucoma" Brit J Ophthalmol. 80-8. 750-754 (1996)

Toshiaki Kubota、Akihiko Tawara 等人：“新生血管性青光眼眼小梁间隙中的新生血管组织”Brit J Ophthalmol。

Tawara A.,et al.: "Distribution and characterization of proteoglycans associated with exfoliation material." Curr.Eye Res.15・11. 1101-1111 (1996)

Tawara A., et al.：“与剥落材料相关的蛋白多糖的分布和表征。”Curr.Eye Res.15・11 (1996)。

Kaoru Motomatsu, Toshiaki Kubota, Akihiko Tawara: "Correlation between optic disc parameters and visual field in glaucoma by laser scanning tomography" Jpn J Clinic Ophthalmol. 50-4. 525-533 (1996)

Kaoru Motomatsu、Toshiaki Kubota、Akihiko Tawara：“激光扫描断层扫描青光眼视盘参数与视野之间的相关性”Jpn J Clinic Ophthalmol。

Akihiko Tawara, et al.: "Distribution and characterization of proteoglycans associated with exfoliation material." Curr Eye Res. 15-11. 1101-1111 (1996)

Akihiko Tawara 等人：“与剥落材料相关的蛋白多糖的分布和表征。”

Kubota T., Tawara A., et al.: "Neovascular tissue in the intertrabecular spaces in eyes with neovascular glaucoma" Brit. J. Ophthalmol.80・8. 750-754 (1996)

Kubota T., Tawara A., et al.：“新生血管性青光眼眼内小梁间隙中的新生血管组织”Brit. J. Ophasemol.80·8 (1996)。