MOLECULAR BIOLOGIC STUDY ON CORTICOSTEROID-INDUCED GLAUCOMA

对日本大耳白兔右眼分别注射曲安奈德20 mg和40 mg，定期测量眼压，连续4周。取动物的前房角组织，用阿新蓝或铜蓝进行组织学和组织化学检查。与对照左眼相比，术后眼压无明显升高。皮质类固醇注射眼的小梁组织未见组织学或组织化学改变。结果提示，人眼和兔眼对糖皮质激素的反应性可能有所不同。我们烧灼了14只兔右眼的双侧睫状后长动脉。烧灼后1、2、4、7、9、14天摘除眼球，光镜观察。血管内皮细胞生长因子受体…的定位观察组织中血管内皮生长因子(VEGF)蛋白及VEGFmRNA表达的变化。在虹膜前表面和小梁网中可检测到新生血管。视网膜未见组织学改变。血管内皮生长因子表达于虹膜上皮细胞和血管内皮细胞。在睫状体上皮细胞中可检测到VEGF mRNA的表达。这些结果表明，眼前段缺血导致眼前段新生血管的形成。对4例剥脱综合征患者眼球摘除后的小梁组织进行了组织学和免疫组织化学检测。剥离纤维含有硫酸软骨素、硫酸皮肤素和硫酸乙酰肝素型蛋白多糖以及HNK-I表位，而细胞外基质中的微纤维则不含。结果表明，蛋白多糖和其他大分子在微纤维上的沉积可能与剥离物的形成密切相关。我们对包括早产儿和老年受试者在内的摘除的人眼的睫状体带宽度与后缘缘宽度的比率进行了形态学检查，称为理论C/L比率。我们还在临床上检查了虹膜角膜角开放的青光眼患者和年龄匹配的对照组的房角镜下睫状体带的外观。理论C/L比值为1.4(SY+-)。[)早产儿2.7%，24.0()SY+-。[)5.4%的儿童和36.7()SY+-。[]老年组为8.0%。在房角镜检查中，77.8%的青光眼和13.8%的对照眼睫状体束不可见或很窄。结果表明，一条看不见或很窄的睫状体带代表着该角度的间接发育。较少

We performed subtenon injection of 20 or 40 mg triamcinolone acetonide into right eyes of Japanese rabbits, and measured their intraocular pressures periodically for 4 weeks. The anterior chamber angle tissues obtained from the animals were examined histologically and histochemically using alcian blue or cupromeronic Blue. There was no significant increase of the intraocular pressure compared to control left eyes. No histological or histochemical change was detected in the trabecular tissues from corticosteroid injected eyes. The results suggest that there may be some difference in reactivity to corticosteroid between human and rabbit eyes.The effects of anterior segment ischemia on angiogenesis in the anterior segment of the eye were investigated. We cauterized both of the long posterior ciliary arteries in the right eye of 14 rabbits. The eyes were enucleated 1,2,4,7,9 and 14 days after cauterization and were studied with light microscopy. The localization of vascular endothelial gro … More wth factor (VEGF) protein and the expression of VEGF mRNA in the tissues were studied. Neovascularization on the anterior surface of the iris and in the trabecular meshwork was detected. No histological changes were observed in the retina. VEGF protein was detected in the epithelial and vascular cells of the iris. Expression of VEGF mRNA was detected in the epithelial cells of the ciliary body. These results suggest that anterior segment ischemia causes neovascularization in the anterior segment of the eye.The trabecular tissues from 4 enucleated human eyes with exfoliation syndrome were examined histologically and immunohistochemically. Exfoliation fibers were revealed to contain chondroitin sulfate dermatan sulfate and heparan sulfate-type proteoglycans as well as HNK-I epitope, while microfibrils in the extracellular matrix did not. The results suggest that depositions of proteoglycans and other macromolecules on the microfibrils may be closely associated with the formation of exfoliation materials.We examined morphologically the ratio of the ciliary body band width to the width of the posterior limbus, termed the theoretical C/L ratio, of enucleated human eyes, including those from premature infants as well as from older subjects. We also examined clinically the appearance of the ciliary body band on gonioscopy in patients having glaucoma with open iridocorneal angles, and in age mathched controls. The theoretical C/L ratio was 1.4(]SY.+-。[)2.7% in premature infants, 24.0(]SY.+-。[)5.4% in children and 36.7(]SY.+-。[)8.0% in the older subjects. On gonioscopy, 77.8% glaucomatous eyes and 13.8% control eyes showed either an invisible or very narrow ciliary body band. The results indicate that an invisible or very narrow ciliary body band represents an nuderdevelopment of the angle. Less