Roles of Extracellular Matrix in Developmental Mechanism and Treatment of Glaucoma

细胞外基质在青光眼发育机制和治疗中的作用

• 批准号: 12671732
• 负责人: TAWARA Akihiko
• 金额: $ 2.11万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671732/
• 关键词: extracellular matrix; MYOC protein; steroid-induced glaucoma; immunohistochemistry; nipradilol; MMPs; TIMPs; type VI collagen; MMP; TIMP; アクアポリン; 血管新生緑内障; 虹彩ルベオーシス; VI型コラーゲン; 原発開放隅角緑内障

1. We examined immunohistochemically expression of the extracellular matrixes and their metabolizing enzymes in the monkey eyes that were injected triamcinolone acetonide under the tenon capsule to study the effect of corticosteroid on the extracellular matrix in the trabecular meshwork. The results suggested that corticosteroid could increase the amount of MYOC protein in the trabecular meshwork, and that it also could increase expression of TIMP-2 in the trabecular meshwork and MMP-3, MMP-9 and TIMP-2 in the ciliary body.2. We immunohistochemically stained the extracellular matrixes in the trabecular meshwork obtained by trabeculectomy from a patient with corticosteroid-induced glaucoma. The results indicated that MYOC protein might increase in the glaucomatous cornea but not in the trabecular meshwork. The results also showed that both heparan sulphate-type proteoglycan and type IV collagen increase in the trabecular meshwork. With double staining, MYOC protein was co-localized with … More type VI collagen in the tissue.3. We examined immunohistochemically localization of MMPs and TIMPs in the normal rat eyes. The results indicated that MMP-9 and TIMP-1 are localized in the cornea, MMP-9 and TIMP-2 are in the trabecular meshwork, and MMP-9, TIMP-1 and TIMP-3 are in the ciliary body.4. We examined immunohistochemically the effect of anti-glaucoma drug of nipradilol, both α- and β-blocker, on MMPs and TIMPs in rat eyes. From this study, it is indicated that nipradilol increases the amount of MMP-2 and MMP-9, and TIMP-1, and decreases TIMP-2 in the trabecular meshwork.5. We examined histologically, immunohistochemically and with in situ hybridization the trabecular tissue from rabbit with experimental anterior segment ischemia to investigate the mechanism of neovascularization in the anterior segment of the eye. The results suggested that vascular endothelial growth factor (VEGF) plays an important role in development of neovascularization in the anterior segment of the eye. Less

1.本研究通过免疫组化方法检测了眼筋膜下注射曲安奈德后小梁网细胞外基质及其代谢酶的表达，以研究皮质类固醇对小梁网细胞外基质的影响。提示皮质类固醇可增加小梁网MYOC蛋白的表达，增加小梁网TIMP-2和睫状体MMP-3、MMP-9、TIMP-2的表达.我们对皮质类固醇诱导的青光眼患者小梁切除术获得的小梁网细胞外基质进行免疫化学染色。结果表明，MYOC蛋白在青光眼角膜中可能增加，而在小梁网中不增加。结果还表明，硫酸乙酰肝素型蛋白多糖和IV型胶原在小梁网中增加。通过双重染色，MYOC蛋白与 ...更多信息 VI型胶原在组织中。我们研究了MMPs和TIMPs在正常大鼠眼中的化学定位。结果表明，MMP-9和TIMP-1定位于角膜，MMP-9和TIMP-2定位于小梁网，MMP-9、TIMP-1和TIMP-3定位于睫状体.我们用化学方法检测了抗青光眼药物尼普拉地洛（nipradilol）对大鼠眼内基质金属蛋白酶（MMPs）和基质金属蛋白酶组织抑制剂（TIMPs）的影响。本研究表明，尼普拉地洛可增加小梁网中MMP-2、MMP-9和TIMP-1的含量，降低TIMP-2的含量.本文对实验性眼前节缺血兔眼小梁组织进行了组织学、免疫组化和原位杂交研究，以探讨眼前节新生血管形成的机制。结果提示，血管内皮生长因子（VEGF）在眼前段新生血管形成中起重要作用。少

项目成果

Tawara A., Kubota T., Hata Y., Sakamoto T.et al.: "Neovascularization in the Anterior Segment of the Rabbit Eye by Experimental Anterior Ischemia"Graefe's Arch Clin Exp Ophtalmol. (印刷中).

Tawara A.、Kubota T.、Hata Y.、Sakamoto T. 等人：“实验性前部缺血引起的兔眼前段新生血管”Graefe 的 Arch Clin Exp Ophtalmol（正在印刷中）。

永谷 建: "正常眼および正常眼圧緑内障眼における視神経乳頭と脈絡膜の循環"眼科臨床医報. 95-11. 1109-1113 (2001)

Ken Nagatani：“正常眼和正常眼压青光眼眼中的视盘和脉络膜的循环”眼科临床杂志 95-11（2001）。

Hirose N., Iwasaki T., Nasu T., Ibi K., Tawara A.: "Effect of a signal instillation of latanoprost on the quasi-static characteristics of accommodation"Jpn J Clin Ophthalmol. 56・6. 957-960 (2002)

Hirose N.、Iwasaki T.、Nasu T.、Ibi K.、Tawara A.：“拉坦前列素的信号滴注对调节的准静态特性的影响”Jpn J Clin Ophamol. 56・6。 2002）

Tawara, A.: "Immunohistochemical localization of MYOC/TIGR protein in the trabecular tissue of normal and glaucomatous eyes"Curr. Eye Res.. 21-6. 934-943 (2000)

Tawara, A.：“正常和青光眼眼小梁组织中 MYOC/TIGR 蛋白的免疫组织化学定位”。

廣瀬 直文: "ラタノプロスト点眼が調節の準静的特性に及ぼす影響"臨床眼科. 56-6. 957-960 (2002)

Naofumi Hirose：“拉坦前列素滴眼液对调节准静态特性的影响”临床眼科 56-6（2002）。