Roles of Extracellular Matrix in Developmental Mechanism and Treatment of Glaucoma

细胞外基质在青光眼发育机制和治疗中的作用

• 批准号: 12671732
• 负责人: TAWARA Akihiko
• 金额: $ 2.11万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671732/
• 关键词: extracellular matrix; MYOC protein; steroid-induced glaucoma; immunohistochemistry; nipradilol; MMPs; TIMPs; type VI collagen; MMP; TIMP; アクアポリン; 血管新生緑内障; 虹彩ルベオーシス; VI型コラーゲン; 原発開放隅角緑内障

1. We examined immunohistochemically expression of the extracellular matrixes and their metabolizing enzymes in the monkey eyes that were injected triamcinolone acetonide under the tenon capsule to study the effect of corticosteroid on the extracellular matrix in the trabecular meshwork. The results suggested that corticosteroid could increase the amount of MYOC protein in the trabecular meshwork, and that it also could increase expression of TIMP-2 in the trabecular meshwork and MMP-3, MMP-9 and TIMP-2 in the ciliary body.2. We immunohistochemically stained the extracellular matrixes in the trabecular meshwork obtained by trabeculectomy from a patient with corticosteroid-induced glaucoma. The results indicated that MYOC protein might increase in the glaucomatous cornea but not in the trabecular meshwork. The results also showed that both heparan sulphate-type proteoglycan and type IV collagen increase in the trabecular meshwork. With double staining, MYOC protein was co-localized with … More type VI collagen in the tissue.3. We examined immunohistochemically localization of MMPs and TIMPs in the normal rat eyes. The results indicated that MMP-9 and TIMP-1 are localized in the cornea, MMP-9 and TIMP-2 are in the trabecular meshwork, and MMP-9, TIMP-1 and TIMP-3 are in the ciliary body.4. We examined immunohistochemically the effect of anti-glaucoma drug of nipradilol, both α- and β-blocker, on MMPs and TIMPs in rat eyes. From this study, it is indicated that nipradilol increases the amount of MMP-2 and MMP-9, and TIMP-1, and decreases TIMP-2 in the trabecular meshwork.5. We examined histologically, immunohistochemically and with in situ hybridization the trabecular tissue from rabbit with experimental anterior segment ischemia to investigate the mechanism of neovascularization in the anterior segment of the eye. The results suggested that vascular endothelial growth factor (VEGF) plays an important role in development of neovascularization in the anterior segment of the eye. Less

1。我们检查了细胞外基质的免疫组织化学表达及其在猴子胶囊下注入丁香霉素乙烯酰胺中的猴子眼中的代谢酶，以研究皮质类固醇对小臂丝网中细胞外基质的影响。结果表明，皮质类固醇可以增加小梁网中的肌酸蛋白的量，并且它也可以增加小梁网中TIMP-2的表达，以及纤毛体中MMP-3，MMP-9和TIMP-2的表达。2。我们通过小梁切除术从患有皮质类固醇引起的青光眼的患者从小梁切除术获得的小梁网中的小梁网中进行免疫组织化学染色。结果表明，Myoc蛋白可能会增加青光眼的角膜，而小梁网中的蛋白质可能不会增加。结果还表明，硫酸乙酰肝素型蛋白聚糖和小梁网中IV型胶原蛋白的增加。通过双染色，Myoc蛋白与组织中更多的VI胶原蛋白共定位。3。我们检查了正常大鼠眼中MMP和TIMP的免疫组织化学定位。结果表明，MMP-9和TIMP-1位于角膜，MMP-9和TIMP-2中，在小梁网中，MMP-9，TIMP-1和TIMP-3在睫状体中。4。我们从大鼠眼中的MMP和TIMP中检查了免疫组织化学研究的免疫组织化学的抗云糖瘤药物对MMP和TIMP的影响。从这项研究中可以表明，尼哌二醇增加了MMP-2和MMP-9和TIMP-1的量，并增加了小梁网中的TIMP-2。5。我们通过组织学，免疫组织化学和原位杂交研究了兔子的小梁组织具有实验性抗段缺血，以研究眼前部分中新血管形成的机制。结果表明，血管内皮生长因子（VEGF）在眼前片段的新血管形成中起重要作用。较少的

项目成果

Tawara A., Kubota T., Hata Y., Sakamoto T.et al.: "Neovascularization in the Anterior Segment of the Rabbit Eye by Experimental Anterior Ischemia"Graefe's Arch Clin Exp Ophtalmol. (印刷中).

Tawara A.、Kubota T.、Hata Y.、Sakamoto T. 等人：“实验性前部缺血引起的兔眼前段新生血管”Graefe 的 Arch Clin Exp Ophtalmol（正在印刷中）。

永谷 建: "正常眼および正常眼圧緑内障眼における視神経乳頭と脈絡膜の循環"眼科臨床医報. 95-11. 1109-1113 (2001)

Ken Nagatani：“正常眼和正常眼压青光眼眼中的视盘和脉络膜的循环”眼科临床杂志 95-11（2001）。

Hirose N., Iwasaki T., Nasu T., Ibi K., Tawara A.: "Effect of a signal instillation of latanoprost on the quasi-static characteristics of accommodation"Jpn J Clin Ophthalmol. 56・6. 957-960 (2002)

Hirose N.、Iwasaki T.、Nasu T.、Ibi K.、Tawara A.：“拉坦前列素的信号滴注对调节的准静态特性的影响”Jpn J Clin Ophamol. 56・6。 2002）

廣瀬 直文: "ラタノプロスト点眼が調節の準静的特性に及ぼす影響"臨床眼科. 56-6. 957-960 (2002)

Naofumi Hirose：“拉坦前列素滴眼液对调节准静态特性的影响”临床眼科 56-6（2002）。

Nagaya K., Tawara A., Takahashi H., Fujii H.: "Microcirculation in the optic nerve head and the choroid in eyes with or without normal-tension glaucoma"Jpn Rev Clin Ophthalmol. 95・11. 1109-1113 (2001)

Nagaya K.、Tawara A.、Takahashi H.、Fujii H.：“患有或不患有正常眼压性青光眼的眼睛中的视神经乳头和脉络膜的微循环”Jpn Rev Clin Ophamol.1109-1113（2001） ）