Roles of Extracellular Matrix in Developmental Mechanism and Treatment of Glaucoma

1.采用免疫组织化学方法，观察曲安奈德对小梁网细胞外基质及其代谢酶表达的影响。结果提示：1.糖皮质激素可增加小梁网MYOC蛋白的表达，增加小梁网TIMP-2和睫状体MMP3、MMP9、TIMP2的表达。我们用免疫组织化学方法对1例皮质类固醇诱发青光眼患者小梁切除后的小梁细胞外基质进行了染色。结果表明，MYOC蛋白在青光眼角膜中可能增加，但在小梁网中不增加。结果还显示，小梁网中硫酸乙酰肝素型蛋白多糖和IV型胶原均增加。通过双重染色，MYOC蛋白与…共定位组织中VI型胶原较多。我们用免疫组织化学方法检测了正常大鼠眼内MMPs和TIMPs的定位。结果表明，基质金属蛋白酶-9和基质金属蛋白酶-1定位于角膜，基质金属蛋白酶-9和基质金属蛋白酶-2位于小梁网，基质金属蛋白酶-9、组织型基质金属蛋白酶-1和基质金属蛋白酶-3位于睫状体。我们用免疫组织化学方法检测了抗青光眼药物尼普拉地洛(α和β阻断剂)对大鼠眼部基质金属蛋白酶和基质金属蛋白酶的影响。本研究表明，尼普拉地洛可增加小梁网中基质金属蛋白酶-2和基质金属蛋白酶-9及TIMP-1的表达，降低TIMP-2的表达。应用组织学、免疫组织化学和原位杂交技术对实验性眼前段缺血兔眼的小梁组织进行组织学、免疫组织化学和原位杂交观察，以探讨眼前段新生血管形成的机制。结果提示，血管内皮生长因子在眼前段新生血管的形成中起重要作用。较少

1. We examined immunohistochemically expression of the extracellular matrixes and their metabolizing enzymes in the monkey eyes that were injected triamcinolone acetonide under the tenon capsule to study the effect of corticosteroid on the extracellular matrix in the trabecular meshwork. The results suggested that corticosteroid could increase the amount of MYOC protein in the trabecular meshwork, and that it also could increase expression of TIMP-2 in the trabecular meshwork and MMP-3, MMP-9 and TIMP-2 in the ciliary body.2. We immunohistochemically stained the extracellular matrixes in the trabecular meshwork obtained by trabeculectomy from a patient with corticosteroid-induced glaucoma. The results indicated that MYOC protein might increase in the glaucomatous cornea but not in the trabecular meshwork. The results also showed that both heparan sulphate-type proteoglycan and type IV collagen increase in the trabecular meshwork. With double staining, MYOC protein was co-localized with … More type VI collagen in the tissue.3. We examined immunohistochemically localization of MMPs and TIMPs in the normal rat eyes. The results indicated that MMP-9 and TIMP-1 are localized in the cornea, MMP-9 and TIMP-2 are in the trabecular meshwork, and MMP-9, TIMP-1 and TIMP-3 are in the ciliary body.4. We examined immunohistochemically the effect of anti-glaucoma drug of nipradilol, both α- and β-blocker, on MMPs and TIMPs in rat eyes. From this study, it is indicated that nipradilol increases the amount of MMP-2 and MMP-9, and TIMP-1, and decreases TIMP-2 in the trabecular meshwork.5. We examined histologically, immunohistochemically and with in situ hybridization the trabecular tissue from rabbit with experimental anterior segment ischemia to investigate the mechanism of neovascularization in the anterior segment of the eye. The results suggested that vascular endothelial growth factor (VEGF) plays an important role in development of neovascularization in the anterior segment of the eye. Less