Innervation of blood vessels in the dental pulp -a morphological study-

用结缔组织消化后的扫描电镜观察牙髓血管的神经支配模式，用多克隆抗pgp和抗P物质抗体免疫组化定位后的透射电镜观察牙髓血管的神经支配模式。扫描电镜显示，无髓神经纤维穿过髓中心血管的外膜，然后进入小动脉的中膜。神经纤维分成许多支索，这些支索终止于平滑肌细胞和内皮细胞的表面。末梢纤维到达成骨下毛细血管丛并终止于周细胞。用多克隆抗pgp抗体和抗P物质抗体免疫电镜也证实了相同的结果。本研究表明，牙髓内的微血管和大血管均受自由神经末梢的直接支配，表明局部血管对血流的调节不仅发生在大血管上，也可能发生在该组织的微血管水平上。

The innervation pattern of blood vessels in the dental pulp was investigated by scanning electron microscopy after connective tissue digestion and by transmission electron microscopy after immunohistochemical localization with polyclonal anti-PGP and anti-suhstance P antibodies. SEM specimens revealed that unmyelinated nerve fibers passed through the tunica adventitia of the blood vessels in the center of the pulp and that these fibers then entered the tunica media of the smaller arterioles. The nerve fibers divided into many collateraIs, and these terminated on the surface of smooth muscle cells and endothelial cells. The terminal fibers then reached the subodontoblastic capillary plexus and terminated on pericytes. The same results were confirmed by immunoelectron microscopy both with polyclonal anti-PGP and anti-substance P antibodies. The present study demonstrated that in the dental pulp the microvasculature as well as larger vessels were directly innervated by free nerve endings, suggesting that the local regulation of blood vessels of blood flow could take place not only at larger vessels but also at the level of the microvasculature in this tissue.