Apoptosis in neuronal cell death of neurodegenerative disorders.

帕金森病患者黑质神经元细胞死亡的原因和机制尚不清楚，细胞凋亡是否在帕金森病患者神经元死亡中起作用存在争议。我们采用原位末端标记法在死后脑黑质中观察PD和进行性核上性麻痹（PSP）患者黑质中的凋亡样小体。对照组、PD和PSP大鼠脑内可见凋亡样小体。凋亡样小体的数量与死亡年龄或死后时间无相关性。细胞凋亡样小体的平均数目在对照组、PD组和PSP组之间无显著差异。此外，对被认为是细胞凋亡早期标志的ss-DNA的免疫组化结果显示，PD组与对照组的ss-DNA阳性神经元数量无显著差异。然而，Bcl-2家族的免疫组化显示，PD中bcl - xl阳性神经元的比例明显低于对照组，尽管Bcl-2和bax阳性神经元的比例在PD与对照组之间没有差异。Bcl-xL的表达降低可能与PD黑质神经元的易损性有关。

The cause and mechanism of neuronal cell death in the substantia nigra of patients with Parkinson disease (PD) is unknown and there is controversy whether apoptosis play a role in the neuronal death in PD.We investigated apoptotic-like body in the substantia nigra of PD, and progressive supranuclear palsy (PSP) using In situ end labeling method on the substantia nigra of the postmortem brain. Apoptotic-like bodies were seen in the brains of the control , PD and PSP.There were no correlation between the number of apoptotic-like bodies and age at death or the postmortem time. The mean number of apoptotic-like bodies were not significantly different between control, PD and PSP.In addition, the inimunohistochemistry against ss-DNA, which are thought as early marker of the apoptosis, showed no significant difference between PD and control in the number of ss-DNA positive neurons.However, the immunohistochemistry for the Bcl-2 family showed the proportion of Bcl-xL-positive neurons was significantly lower in PD than control, although the proportions of Bcl-2 and Bax-positive neurons were not different between PD and control. Reduced expression of Bcl-xL may play a role in the vulnerability of the neurons in the substantia nigra of PD.