Development of the screening system for a new drug which modifies the Rho small GTP-binding protein-mediated signaling pathway

Rho小gtp结合蛋白参与了癌细胞的侵袭和转移。酿酒酵母也具有Rho信号通路，这表明酿酒酵母可以用来寻找一种新的药物来阻止癌细胞的侵袭和转移。在本研究中，我们试图建立这样一个系统，并研究Rho在酵母中的作用模式。Rho的作用方式：BNI1和BNR1是Rho小gtp结合蛋白的潜在靶点，并与多种细胞骨架蛋白相互作用，包括伸长因子1- α、SPA2和HOF1，以调节细胞骨架系统。筛选系统的开发：哺乳动物细胞中的Rho和Rho相关基因在酵母中表达，发现这些基因的表达可以抑制酵母细胞的生长，这表明可以利用这些酵母细胞筛选出一种影响Rho信号通路的新药。

The Rho small GTP-binding protein is implicated in the invasion and metastasis of the cancer cells. The yeast Saccharomyces cerevisiae also possesses the Rho signaling pathway, suggesting that S.cerevisiae can be used to search for a new drug which prevents the invasion and metastasis of the cancer cells, In this study, we have attempted to establish such a system and investigated the modes of action of Rho in yeast.Modes of action of Rho : BNI1 and BNR1 are potential targets of Rho small GTP-binding protein and interact with a variety of cytoskeletal proteins, including Elongation factor 1-alfa, SPA2, and HOF1, to regulate the cytoskeletal system.Development of the screening system : Rho and Rho-related genes in mammalian cells were expressed in yeast and it was found that the expression of these genes prevents the growth of yeast cells, indicating that these yeast cells can be used to screen out a new drug which affects the Rho-signaling pathway.