Establishment of Supporting System for evaluating Biological Environments based on Protistan DNA nucleotide sequences

为了建立海洋生物环境评价的支持系统，建立了核糖体DNA序列的原生生物遗传数据库。DNA分析。对核糖体DNA的LSU、SSU和ITS序列进行了分析。从静冈大学的出版物和未发表的论文中汇编了物种与环境之间的关系，以提供数据库的生态或形态限制。分析样本收集自日本群岛从北到南的7个内湾。选取6种底栖有孔虫(Protista)进行分析。它们通常被用来作为内湾环境中营养不良环境或富营养化条件的替代物。列出了每个物种的序列数据，以及种内和/或种间差异，作为一个数据集。…汇编了对环境变化的更多生理、生态和形态反应，以将每个物种描述为特定的环境替代物。例如，生活在微咸水海湾富营养化环境中的TroChammina hadai Uchio，会根据沉积物-水界面的溶解氧浓度改变其测试形态。该种在水温10~18℃，盐度25~30‰，溶解氧浓度6~8ml/L的条件下繁殖，其序列数据具有足够的独特性，足以区别于类似的类群。LSU rDNA序列在不同分布区之间仅有0.1%的差异。ITS区具有2.0%的序列差异，ITS区是发生频繁替换的区域，在有机碳沉积严重的高污染海域，序列差异往往小于污染较轻的区域。这一趋势可以作为一个很好的指标来评估因污染而发生的精确环境变化。较少

In order to establish a support system for assessing marine biological environments, a genetic database of protistan species, which are commonly used as environmental proxies, was constructed for ribosomal DNA sequences. DNA analyses. were carried out for LSU, SSU and ITS sequence regions of ribosomal DNA. Relationships between species and ambient environments were compiled, both from publications and unpublished theses of Shizuoka University, to provide ecological or morphological constraints for the database.Samples for analyses were collected from seven innerbays from north to south along the Japanese Islands. Six species of benthic foraminifera (Protista) were selected for analysis. All are commonly used as proxies for dysaerobic environment or eutrophicated condition within the inner bay environment.The database was constructed for DNA sequences of these species. Sequence data for each species were listed, together with intraspecific and/or interspecific divergences, as a dataset. … More Physiological, ecological and morphological responses to ambient environmental changes were compiled to characterize each species as a specific environmental proxy. For instance, Trochammina hadai Uchio, which dwells in eutrophic environments of brackish bays, changes its test morphology in relation to the dissolved oxygen concentration at the sediment-water interface. This species reproduces at a water temperature of 10〜18 ℃, salinity of 25〜30 ‰, and dissolved oxygen concentration of 6〜8 ml/L. Sequence data for this species are sufficiently unique to discriminate from similar taxa. LSU rDNA sequences show only 0.1% difference between distributional areas. The ITS region, where substitution occurs very frequent, shows 2.0% of sequence differences.Sequence divergence tends to be smaller in highly polluted area of the sea, where heavy deposition of organic carbon takes place, than in less-polluted area. This tendency can be used as a good proxy for assessing precise environmental changes in response to pollution. Less