Search for the bioactive molecules implicated in the regulation of human periodontal ligament cell functions

周期性法律（PDL）具有保持牙齿根接触的关键作用。We found that cultured human PDL cells （hPDL cells） spontaneously produced nitric oxide （NO）。这种“不生产”不受影响，是通过用白细胞素-1 β（IL-1 β）培养细胞、However，而“不生产”则通过刺激细胞的循环张力。在不受刺激的hPDL细胞培养中，集中了NO D22-D2和NO D23-D2 （NO D22-D2/NO D23-D2），氧化物的NO产品，在新更换的介质中，初始值增加到140%。在对比中， NO-D22-D-2/NO-D-23-D-2显示了当细胞被注射到12小时的循环张力力时的3-折叠增加，我们使用了RT-PCR方法来检测Hpdl细胞中的NO合成酶mRNA。Endothelial NO synthase （ecNOS） mRNA was expressed in both stimulated and unstimulated Hpdl cells whereas inducible NO synthase （iNOS） mRNA was detected in neither culturing condition。Furthermore，我们找到了强大的ecNOS，但在刺激的Hpdl细胞中没有iNOS免疫活性。已被机械刺激的Hpdl细胞被建议通过向上调节的无生产来调节周期性组织的功能，NF-卡帕B配体（RANKL）及其decoy受体、骨细胞蛋白（OPG）的受体激活剂的表达被调查于hPDL细胞的文化。Both RANKL and OPG mRNAs were expressed in the hPDL cells and Northern blot analysis revealed that the OPG mRNA expression was down-regulated remarkably by an application of 1 alpha， 25（OH）伊D22伊D2 vitamin D3 （1，25-（OH）伊D22伊D23伊D2） and dexamethasone （Dex）。相比之下， RANKL mRNA表达是由相同的治疗进行的。Tartrate-resistant acid phosphatase-positive multinuclear cells were markedly induced when the hPDL cells were co-cultured with bone marrow cells in the presence of an anti-OPG抗体together with 1，25-（OH）y D22 y D2D y D23 y D2 and Dex.

The periodontal ligament (PDL) has a critical role of keeping the tooth root intact. We found that cultured human PDL cells (hPDL cells) spontaneously produced nitric oxide (NO). This NO production was not affected by culturing the cells with interleukin-1 β(IL-1 β), However, the NO production was enhanced by stimulating the cells with cyclic tension forces. In an unstimulated hPDL cell culture, concentration of NOィイD22-ィエD2 and NOィイD23-ィエD2 (NOィイD22-ィエD2/NOィイD23-ィエD2), the oxidized products of NO, increased to 140% of the initial value during the first 12 h in newly exchanged medium. In contrast, NOィイD22-ィエD2/NOィイD23-ィエD2 showed a 3-fold increase when the cells had been subjected to cyclic tension forces for 12 h.We employed RT-PCR method for the detection of NO synthase mRNA in the Hpdl cells. Endothelial NO synthase (ecNOS) mRNA was expressed in both stimulated and unstimulated Hpdl cells whereas inducible NO synthase (iNOS) mRNA was detected in neither culturing condition. Furthermore, we found strong ecNOS but not iNOS immunoreactivity in the stimulated Hpdl cells. Mechanically stimulated Hpdl cells were suggested to modulate the functions of periodontal tissue by the upregulated NO production.Expression of receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG) was investigated in cultures of hPDL cells. Both RANKL and OPG mRNAs were expressed in the hPDL cells and Northern blot analysis revealed that the OPG mRNA expression was down-regulated remarkably by an application of 1 alpha, 25(OH)ィイD22ィエD2 vitamin D3 (1,25-(OH)ィイD22ィエD2DィイD23ィエD2) and dexamethasone (Dex). In contrast, RANKL mRNA expression was up-regulated by the same treatment. Tartrate-resistant acid phosphatase-positive multinuclear cells were markedly induced when the hPDL cells were co-cultured with bone marrow cells in the presence of an anti-OPG antibody together with 1,25-(OH)ィイD22ィエD2DィイD23ィエD2 and Dex.