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Molecular epidemiology concerned with effect of nitric oxide synthase (NOS) gene polymorphism on NOS protein function in human cultured vascular endothelial cells

一氧化氮合酶（NOS）基因多态性对人培养血管内皮细胞NOS蛋白功能影响的分子流行病学

基本信息

批准号：
14370123
负责人：
KISHIMOTO Takuji
金额：
$ 8.7万
依托单位：
National University Corporation Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14370123/
关键词：
nitric oxide synthase gene polymorphism primary culture endothelial cell protein function molecular epidemiology

项目摘要

The relationship between cardiovascular disease and Glu298Asp polymorphism of the nitric oxide synthase (eNOS) gene in vascular endothelial cells is controversial due to conflicting results. To demonstrate the effects of Glu298Asp polymorphism of the eNOS gene on eNOS gene expression by excluding the effects of various confounding factors, eNOS mRNA and protein expressions were measured using cultured human vascular endothelial cells according to genotypes of Glu298Asp polymorphism of the eNOS gene. After human umbilical cords were aseptically obtained immediately after delivery, human umbilical vein endothelial cells (HUVECs) were collected by collagenase treatment, and HUVECs with the Glu298Glu or Glu298Asp genotype were obtained. eNOS mRNA expression was measured by real-time PCR/RT-PCR. eNOS protein expression was measured by an immunoassay method. HUVECs with Glu298Asp tended to show higher eNOS mRNA and protein expressions than those with Glu298Glu, but the difference was not significant. These results suggest that the genotypic difference of Glu298Glu and Glu298Asp has no effect on eNOS mRNA and protein expressions.

血管内皮细胞一氧化氮合酶（eNOS）基因Glu 298 Asp多态性与心血管疾病的关系存在争议。为了排除各种混杂因素的影响，证明eNOS基因的Glu 298 Asp多态性对eNOS基因表达的影响，根据eNOS基因的Glu 298 Asp多态性的基因型，使用培养的人血管内皮细胞测量eNOS mRNA和蛋白质表达。在无菌条件下获得人脐带后，立即通过胶原酶处理收集人脐静脉内皮细胞（HUVECs），并获得具有Glu 298 Glu或Glu 298 Asp基因型的HUVECs。实时定量PCR/RT-PCR检测eNOS mRNA表达。用免疫分析法测定eNOS蛋白表达。Glu 298 Asp转染的HUVECs eNOS mRNA和蛋白表达均高于Glu 298 Glu转染的HUVECs，但差异无统计学意义。提示Glu 298 Glu和Glu 298 Asp基因型差异对eNOS mRNA和蛋白表达无影响。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

eNOS Glu298Asp polymorphism and hypertension in a cohort study in Japanese

DOI：
10.1016/j.ypmed.2004.03.030
发表时间：
2004-11-01
期刊：
PREVENTIVE MEDICINE
影响因子：
5.1
作者：
Kishimoto, T;Misawa, Y;Fukumoto, S
通讯作者：
Fukumoto, S

Effects of Glu298Asp Polymorphism of Endothelial Nitric Oxide Synthase (eNOS) Gene on eNOS mRNA and Protein Expressions in Cultured Human Vascular Endothelial Cells

内皮一氧化氮合酶(eNOS)基因Glu298Asp多态性对培养人血管内皮细胞eNOS mRNA和蛋白表达的影响

DOI：
发表时间：
2006
期刊：
Yonago Acta medica 49
影响因子：
0
作者：
Takuji Kishimoto;Akihiko Kaetsu;Yoneatsu Osaki;Mikizoh Okamoto;Mariya Nagai;Yoichi Kurosawa;Soichi Yoshida
通讯作者：
Soichi Yoshida

The lack of relationship between an endothelin-1 gene polymorphism (Ala288ser) and incidence of hypertension: a retrospective cohort study among Japanese workers.