A fast fluorescence lifetime imaging microscope to track protein dynamics

该项目旨在建立一种快速荧光寿命成像显微镜，可以在活细胞的整个结构框架中跟踪蛋白质的细胞内旅程。通过将单颗粒追踪技术与尖端的荧光寿命相机相结合，这款独一无二的显微镜将能够以前所未有的时间分辨率在空间上绘制蛋白质迁移率和相互作用。这项技术的好处是，它将使澳大利亚的科学家能够首次成像蛋白质导航细胞内结构以在单分子水平上调节复杂生物功能的生物物理机制。

This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolution. The benefit of this technology is that it will enable scientists in Australia to image, for the first time, the biophysical mechanism by which a protein navigates intracellular architecture to regulate a complex biological function at the single molecule level.