Analytical and Biophysical Research Core

项目摘要/摘要 密西西比大学提议建立血糖科学卓越研究中心 (GlyCORE)，一期生物医学研究卓越中心。血糖科学是一种基本的、快速的 生物医学的新兴领域，而糖科学研究的挑战性需要工具和 在生物医学研究界中不常见的专业知识。此外，生物医学 社区正在迅速发展，认识到碳水化合物的基本作用，研究人员在 自我认同的“糖科学”社区发现自己面临着解决挑战性问题 作为他们生物医学研究人员课程的一部分。分析与生物物理学的焦点 研究的核心是三个方面：在适当的碳水化合物样本中培训新接触糖科学的研究人员 准备和处理；支持假设驱动的研究，从以下方面进行有针对性的定量分析 既有丰富的样本，也有复杂的混合物；支持基于发现的研究，为研究人员提供 目标系统扰动时的系统级更改视图。培训研究人员是关键 核心的职能，特别是在第一阶段，当糖科学方面的机构专门知识水平处于 最早的发展阶段。核心1将培训研究人员碳水化合物和碳水化合物结合物 样品制备技术。有针对性的分析将作为假设驱动的关键技术提供 研究，研究人员想要测试给定的多糖或糖苷的存在和数量， 对未知分子进行结构表征，或验证关键的生物或化学资源。结构性 糖链修饰、蛋白质-碳水化合物相互作用、结合亲和力估计等的分析 生物物理分析将通过基于MS、LC和分光光度的技术得到支持。访问 还将提供核磁共振仪器以及光谱获取和解释方面的专业知识。最后， 核心1号将提供“组学”式的基于发现的糖类、蛋白质组、代谢组等的研究。 定量、半定量和定性方式，支持系统糖生物学和假说 针对靶向分析设计的开发。核心1将与计算化学和 生物信息学核心，为“组学”数据提供先进的统计和路径分析。的服务 CORE 1将使用密西西比大学现有的资源以及专用的高分辨率Thermo Q-Exactive Plus LC-MS/MS系统和Dionex Ultal3000分析UHPLC系统。由于穷人 大多数碳水化合物的紫外吸收特性，标记碳水化合物的荧光检测和 对未衍生碳水化合物的带电气溶胶检测也将用于灵敏检测和 在高动态范围内进行定量。通过将我们的第一阶段分析和生物物理核心集中在一个 许多灵活的技术，我们可以支持UM调查人员不同的血糖科学兴趣 使用有限一系列设备的尖端技术，并随着GlyCORE的成熟有增长的空间。

Project Summary/Abstract The University of Mississippi proposes to establish the Glycoscience Center of Research Excellence (GlyCORE), a Phase I Center of Biomedical Research Excellence. Glycoscience is an essential and rapidly emerging field of biomedical science, and the challenging nature of glycoscience research requires tools and expertise that are not commonly found in the biomedical research community. Moreover, the biomedical community is rapidly growing to appreciate the essential role of carbohydrates, with researchers outside of the self-identified “glycoscience” community finding themselves faced with addressing challenging questions in glycoscience as part of the course of their biomedical researchers. The focus of the Analytical and Biophysical Research Core is three-fold: to train investigators new to glycoscience in proper carbohydrate sample preparation and handling; to support hypothesis-driven research with targeted quantitative analyses from both enriched samples and complex mixtures; and to support discovery-based research to give investigators a system-level view of changes upon perturbation of targeted systems. Training of researchers is a key function of the Core, especially in Phase I when the institutional level of expertise in glycoscience is in its earliest stage of development. Core 1 will train researchers in carbohydrate and carbohydrate conjugate sample preparation techniques. Targeted analyses will be provided as a key technology for hypothesis-driven research, where an investigator wants to test for the presence and quantity of a given glycan or glycoside, structurally characterize an unknown molecule, or validate a key biological or chemical resource. Structural analyses of glycan modification, protein-carbohydrate interactions, binding affinity estimations, and other biophysical analyses will be supported through MS-, LC- and spectrophotometry-based technologies. Access to NMR instrumentation and expertise in spectral acquisition and interpretation will also be provided. Finally, Core 1 will offer “omics”-style discovery-based surveys of glycomes, proteomes, metabolomes, etc. in quantitative, semi-quantitative, and qualitative fashions, supporting systems glycobiology and hypothesis development for targeted assay design. Core 1 will interface closely with the Computational Chemistry and Bioinformatics Core to provide advanced statistical and pathway analyses for “omics” data. The services of Core 1 will use both existing University of Mississippi resources as well as a dedicated high resolution Thermo Q-Exactive Plus LC-MS/MS system and a Dionex Ultimate 3000 analytical UHPLC system. Due to the poor UV-absorption characteristics of most carbohydrates, fluorescence detection for tagged carbohydrates and charged aerosol detection of underivatized carbohydrates will also be available for sensitive detection and quantitation at high dynamic range. By focusing our Phase I Analytical and Biophysical Core on a small number of flexible technologies, we can support the diverse glycoscience interests of UM investigators with cutting-edge technology using a limited array of equipment and have room for growth as GlyCORE matures.