Enhancing the Capacity and Capability of Human Food and Environmental Testing Laboratory at the Texas Department of State Health Services: Special Projects Method Development and Method Validation

项目摘要：D学科特别项目 跟踪3-方法开发/验证，qPCR筛查的多个实验室验证 食品中沙门氏菌的检测方法 德克萨斯州卫生服务部实验室服务科(LSS) 一直是食品和药物管理局(FDA)食品应急响应的成员 FERN和基因组Trakr网络，疾病中心成员 控制和预防(CDC)实验室反应网络(LRN)、PulseNet和CaliciNet。 食品安全监督管理局还通过了国际标准化组织/国际电工委员会17025认证，对沙门氏菌、李斯特氏菌、大肠杆菌等10种食品进行了检测。 O157：H7，弯曲杆菌，和大的“6”非O157 STEC物种，接收和分析 每年大约有2500个食物样本。作为综合食品安全体系的一部分， LSS致力于促进和改善食品检测监督、检查和 德克萨斯州的调查。 该项目的目标是参与初步的、短期的或探索性的多实验室 验证(MLV)研究，重点测试FDA定量聚合酶链式反应筛查方法的可行性 用于鉴定食品中的沙门氏菌。LSS高级分子微生物学小组(AMMG) 将遵循FDA指定的最新FDA协议和流程。有两个人 ABI 7500快速实时PCR系统，AMMG可以检测多达1000种食品和沙门氏菌 除所有例行和维护活动外，每年还提供环境样本 预计在执行能力方面不会有任何问题参与MLV研究。有了这些资金 从这笔赠款中，LSS将购买参与MLV研究所需的用品， 培训员工，进行研究，参加与研究有关的会议和活动，以及 维护完成拟议工作所需的设施和人员。 这项合作协议提供的资金将有助于加强我们各州的承诺 提供安全的食品供应，提高防疫体系应对大规模食品安全威胁的能力 国家的粮食供应。

Project Summary: Discipline D Special Projects Track 3- Method Development/Validation, Multi-laboratory Validation of a qPCR Screening Method for Salmonella in Foods The Texas Department of State Health Services (DSHS) Laboratory Services Section (LSS) has been a member of the Food and Drug Administration (FDA) Food Emergency Response Network (FERN) and the Genome Trakr Network and a member of the Centers for Disease Control and Prevention (CDC) Laboratory Response Network (LRN), PulseNet, and CaliciNet. LSS is also ISO/IEC 17025 accredited for ten food tests that cover Salmonella, Listeria, E. coli O157:H7, Campylobacter, and the Big “6” non-O157 STEC species, receiving and analyzing approximately 2,500 food samples per year. As a part of an integrated food safety system, the LSS is committed to facilitating and improving food testing surveillance, inspection, and investigation in the State of Texas. The goal of this project is to participate in preliminary, short-term, or exploratory multi-laboratory validation (MLV) studies that focus on testing the feasibility of the FDA qPCR Screening Method for identifying Salmonella in foods. The LSS Advanced Molecular Microbiology group (AMMG) will follow the most up-to-date FDA protocols and processes as specified by the FDA. With two ABI 7500 Fast Real-Time PCR systems, the AMMG can test up to 1000 Salmonella food and environmental samples per year in addition to all routine and maintenance activities and does not expect any issues in performance capacity to participate in the MLV studies. With the funds provided from this grant, LSS will purchase supplies needed to participate in the MLV studies, train staff, perform the studies, participate in meetings and activities related to the studies, and maintain facilities and personnel necessary to complete the work proposed. Funding provided by this cooperative agreement will serve to strengthen our states commitment to a safe food supply and improve the ability of DSHS to respond to a large-scale threat to the nation's food supply.