Animal Research Equipment, Digital Cages & Metabolic, Avoidance, Fear Conditioning, Place Preference, Self-Administration, Open Field, & Microdialysis Systems for Translational Neuroscience

项目摘要 神经科学的进步依赖于可靠的工具和技术，包括进行功能性研究的能力。 体内研究。BFNL是在斯坦福大学神经科学研究中心内创建的， 重要的行为，翻译和神经药理学资源及其传播到更广泛的 研究社区。来自斯坦福大学和医学院的500万美元的财政支持 在2007年成立民族解放力量之初，启动预算帮助了该力量的建立。BFNL战略装备 与资源提供关键支持，以最广泛的研究项目，并提供承包 为斯坦福大学和国家实验室提供服务和技术。BFNL提供了实验性的工具， 决定大脑在健康和疾病中的功能。这些实验工具可供科学家使用， 无法以其他方式获得这些资源和专业知识作为运行所需的资源成本， 分析行为研究是很重要的BFNL是标准和自动化行为的集中资源 范例，新型转基因品系的最先进的表型分析服务，以及经验证的啮齿动物的专业知识 CNS疾病模型，用于在转化研究中测试新型神经药理学。BFNL是一个独特的， 经过验证的转化神经科学资源，可供斯坦福大学社区以及感兴趣的 全国各地的研究人员为200个实验室和40多个实验室提供了有记录的支持 出版物。根据这一建议，我们将在我们的设备组合中增加以下仪器：240 Digilab-数字通气笼，40个代谢笼，带E-Chiller，8+4 FreezeFrame恐惧条件反射 系统，4位置偏好系统，4回避系统，8自我管理系统，8微透析 和注射泵系统，以及8个开放式野外活动系统。民族解放阵线有着良好的信誉和记录 维护和运行动物行为和药理学设备的专业知识使其处于有利地位， 获得、分发、促进和传播所需设备的使用，以供神经科学界使用 无论是在斯坦福大学还是在其他地方，都以一种独特的方式确保广泛的使用，这将导致巨大的成功 加速转化研究。在过去的13年里，BFNL支持了众多斯坦福大学 实验室与他们的中枢神经系统疾病和翻译的需要，导致治疗的发展和临床 翻译. BFNL一直致力于支持来自卓越实验室的创新， 作为Südhof，Shatz，Barres和Deisseroth，他们已经获得了着名的诺贝尔奖，Keio和NAS 奖品斯坦福大学的许多其他实验室也受益于BFNL的服务和专业知识， 斯坦福大学和全国各地。为了继续支持和推进NIH资助的神经科学研究， BFNL必须继续提供最先进的技术。该申请要求资助9件 在BFNL放置的设备。所要求的设备将支持啮齿类动物的专门工作流程 行为和神经药理学测试，并补充现有的核心设施设备。

Project Summary Advances in neuroscience depend on reliable tools and technologies, including the ability to carry out functional in vivo studies. BFNL was created within the Stanford Neuroscience Research Center to develop and centralize vital behavioral, translational, and neuropharmacological resources and their dissemination to a broader research community. Financial support from Stanford University and School of Medicine in the amount of 5 MM start-up budget helped with the establishment of BFNL at its inception in 2007. BFNL is strategically equipped with resources to provide critical support to the broadest range of research projects and to provide contracted services and techniques to Stanford and national laboratories. BFNL provides experimental access to tools to determine the brain function in health and disease. These experimental tools are available to scientists who may not otherwise have access to these resources and expertise as the cost of resources necessary to run and analyze behavioral studies is substantial. BFNL is a centralized resource for standard and automated behavioral paradigms, state-of-the-art phenotyping services for novel transgenic lines, and expertise in the validated rodent CNS Disease Models for testing novel neuropharmacology in translational studies. BFNL is a unique and validated translational neuroscience resource that is available to the Stanford community as well as interested researchers across the country with a documented history of support for 200 laboratories and more than 40 publications. Under this proposal, we will add the following instrumentation to our portfolio of equipment: 240 Digilab-Digital Ventilated Cages, 40 Metabolic cages with E-Chiller, 8+4 FreezeFrame Fear Conditioning System, 4 Place Preference System, 4 Avoidance System, 8 Self-Administration System, 8 Microdialysis and Syringe Pump System, and 8 Open Field Activity System. BFNL’s well-established and documented expertise to maintain and run animal behavioral and pharmacological equipment makes it well-positioned to obtain, distribute, facilitate, and disseminate usage of the requested equipment to the Neuroscience community both at Stanford and beyond in a unique way that ensures widespread usage that will lead to massive success and acceleration of translational studies. In the past 13 years, BFNL has supported numerous Stanford laboratories with their CNS disease and translational needs, leading to therapeutic development and clinical translation. BFNL has been instrumental in supporting innovations coming from extraordinary laboratories such as Südhof, Shatz, Barres, and Deisseroth, who have been recognized with the prestigious Nobel, Keio, and NAS prizes. Many other laboratories at Stanford have also benefited from BFNL’s services and expertise both at Stanford and across the country. To continue supporting and advancing NIH-funded neuroscience research, BFNL must continue to provide state-of-the-art technologies. This application requests funding for nine pieces of equipment to be placed in BFNL. The requested equipment will support a specialized workflow for rodent behavioral and neuropharmacological testing and complement the already existing core facility equipment.