Carbon nanotube integrated microdevice for next generation sequencing based virus discovery

项目总结 传染病占全球死亡和巨大经济损失的四分之一。病原体发现 在抗击传染病方面至关重要。然而，临床上有很大比例的病原体 传染病病例仍未确定。通常发生在资源有限的地区，主要的疫情是 由新出现的病毒引起，主要是野生动物起源的。病毒感染性疾病面临的紧迫挑战 疾病需要一个样本到答案的病毒发现平台，可以从 环境和临床样本，用于基于高通量测序的病毒发现。在本建议中 在研究方面，我们将开发一种新型的碳纳米管三维样品制备和浓缩微设备(CNT- 3D-SPEM)使用工程碳纳米管材料，如我们的初步研究所示，进入了一个新的 用于病毒颗粒浓缩的连续流动微流控设计。新的微流控设计具有独特的自身 调节流动机制，实现高病毒捕获率、高样本容量和高流速 同时。浓缩后，捕获的病毒被裂解在CNT-3D-SPEM中。病毒基因组 将被提纯并原位扩增，这是通过 高通量测序。与合作调查人员合作，我们将进行多项试点研究， 使用开发的技术从临床和环境样本中发现病毒。最终目标是 开发整个高性能便携平台，推进病毒发现，与病毒作斗争 传染病。

PROJECT SUMMARY Infectious diseases account for one quarter of global deaths and massive economic loss. Pathogen discovery is critical in combating infectious diseases. However, the causative agents in a large percentage of clinical infectious disease cases remain unidentified. Often occurring in resource-limited areas, major outbreaks were caused by newly emerging viruses, mostly wildlife-originated. The pressing challenges in viral infectious diseases call for a sample-to-answer virus discovery platform that can prepare viral samples from environmental and clinical samples for high throughput sequencing based virus discovery. In this proposed research, we will develop a new carbon nanotube 3D sample preparation and enrichment microdevice (CNT- 3D-SPEM) that employs engineered carbon nanotube material, as shown in our preliminary study, into a new continuous flow microfluidic design for virus particle enrichment. The new microfluidic design has a unique self- regulating flow mechanism to achieve high virus capture efficiency and high sample capacity and flow rate simultaneously. After enrichment, the captured viruses are lyzed inside the CNT-3D-SPEM. The viral genome will be purified and amplified in situ, which are required sample preparation steps for virus discovery through high throughput sequencing. Working with co-investigators together, we will conduct multiple pilot studies that use the developed technology for virus discovery from clinical and environmental samples. The ultimate goal is to develop the entire high-performance portable platform to advance virus discovery for the battle with viral infectious diseases.