Behavioral Core

项目概要：行为光遗传学核心 催产素释放和小鼠社会空间行为的光遗传学控制是这个大脑启动的基础 关于“催产素对神经回路功能和行为的调节”的建议。每个项目都需要光学 催产素神经元的鉴定，催产素系统的光学激活或抑制，或其它转基因 表达理解社会行为的神经回路基础。最近的一些技术进步 现在，跨层次的研究能够更深入地理解神经调节，以实现灵活的信息处理， 小鼠的行为。具体地说，现在可以监测和操纵特定的细胞类型（例如，各种 抑制性中间神经元或皮质下调节系统，包括催产素），利用转基因 与病毒方法组合的小鼠系，用于表达视蛋白、荧光团、药物遗传学构建体， 或者用shRNA敲除基因。这些转基因和病毒在小鼠中的方法可以与成像相结合 或在行为头部固定的小鼠中的细胞内记录，在自由移动的小鼠中的四极管或光度测定法，或 在大脑切片中，更深入地了解调制和可塑性的细胞和突触机制。 因此，本行为核心的目标1是繁殖和维持有价值的转基因小鼠品系， 项目实验室（通常用于局部回路的细胞类型特异性操作，某些受体表达细胞，或 远程调节投射）。目标2是通过病毒注射为项目团队实验室使用准备这些动物 （主要是视蛋白、DREADD或荧光报告基因如GCaMP 6）。这将大大加快研究 由项目团队执行，并帮助进行故障排除和资源/试剂验证和复制 跨实验室目标3是与团队实验室成员合作进行行为实验。这是由以下因素促成的： 将BRAIN倡议的行为光遗传学核心纳入纽约大学现有的啮齿动物行为核心， 医学院，增强该核心设施的能力，以改善科学环境 整个机构。

Project Summary: Behavioral Optogenetics Core Optogenetic control of oxytocin release and mouse socio-spatial behavior is fundamental to this BRAIN Initative proposal on “Oxytocin Modulation of Neural Circuit Function and Behavior”. Each Project requires optical identification of oxytocin neurons, optical activation or suppression of the oxytocin system, or other transgene expression for understanding the neural circuit basis of social behavior. A number of recent technical advances across levels now enable a deeper understanding of neuromodulation for flexible information processing and behavior in mice. Specficially, it is now possible to monitor and manipulate specific cell types (e.g., various inhibitory interneurons or subcortical modulatory systems including oxytocin), taking advantage of transgenic mouse lines combined with viral approaches for expressing opsins, fluorophores, pharmacogenetic constructs, or gene knockdown with shRNA. These transgenic and viral approaches in mice can be combined with imaging or intracellular recordings in behaving head-fixed mice, tetrode or photometry methods in freely-moving mice, or in brain slices for deeper understanding of the cellular and synaptic mechanisms of modulation and plasticity. Thus Aim 1 of this Behavioral Core is to breed and maintain valuable transgenic mouse lines, shared between Project labs (generally for cell-type specific manipulation of local circuits, certain receptor-expressing cells, or long-range modulatory projections). Aim 2 is to prepare these animals for Project team lab use via viral injection (mostly of opsins, DREADDs, or fluorescent reporters such as GCaMP6). This will greatly accelerate the studies performed by Project teams, as well as aid in troubleshooting and resource/reagent validation and replication across labs. Aim 3 is to work with team lab members to perform behavioral experiments. This is facilitated by incorporating this BRAIN Initiative Behavioral Optogenetics Core into the existing Rodent Behavior Core at NYU School of Medicine, augmenting the capabilities of that core facility to enhance the scientific environment throughout the institution.