Single-session Introduction of Mutations in Parallel Lines (SIMPL)

该项目旨在开发一种新方法，显著加快转基因小鼠的生产，这是研究生物过程和疾病的关键“工具”。这项工作计划将最新的基因编辑技术CRISPR提升到一个新的水平，通过开发一种基于CRISPR的新方法，以目前成本和时间的一小部分，产生具有相同基因序列的不同变异的不同小鼠品系。这个项目将克服一个主要的障碍，以前所未有的细节研究基因功能，从而为未来的生物过程研究开辟新的途径。因此，这个项目的成果应该会影响整个生物医学研究领域，并推动澳大利亚的生物技术产业。

This project aims to develop a novel method for markedly accelerating production of genetically modified mice, which are a key 'tool' for studying biological processes and diseases. The work plans to take CRISPR, the latest gene-editing technique, to the next level by developing a novel CRISPR-based method to generate different mouse strains with distinct variations of the same gene sequences, at a fraction of the present cost and time. This project should overcome a major barrier to studying gene function with unprecedented detail, thereby opening new avenues for future research into biological processes. Thus, the outcomes from this project should impact on the entire field of biomedical research, and advance Australia's biotech industry.