Lakeside Conference on Protein Toxins and Effectors 2021

项目摘要/摘要 蛋白质毒素和效应物是许多细菌病原体的主要毒力因子。它们通过以下方式引起疾病 瞄准并扰乱关键的细胞功能。从历史上看，对毒素/效应物的研究只集中在 少数几种主要毒素，在建立其机制框架方面取得了巨大成功 在根除许多毁灭性的传染病方面。在这些早期成功的基础上，这个领域已经消失了 通过过去20年的伟大复兴和扩张，从狭隘地专注于几个 将人类疾病扩展到更广泛的生物学领域，重点是理解“以蛋白质为基础”的斗争和- 共同进化的生物体之间的防御通讯。例如，微生物基因组学的最新进展 基于发现的筛选方法导致毒素和效应物的数量急剧增加 正在研究的系统。新的研究还揭示了微生物分泌和注入毒素和效应物 不仅在致病过程中靶向真核细胞，而且参与细菌间的竞争。 高分辨率显微镜、结构生物学、低温电子显微镜、蛋白质组学、 和CRISPR迅速推进了我们对毒素/效应物作用的理解。尽管两者都在增长，但 该领域的深度和广度，目前在北美还没有以毒素/效应物为中心的会议 可以将来自不同领域的人聚集在一起。为了弥补这一差距，我们正在推出一个重新配置的 重塑“面对面-虚拟”杂交会议--“湖畔蛋白质毒素与效应器会议” (#Lakeside PTE)将于2021年10月3日至6日在美国威斯康星州日内瓦湖的修道院度假村举行，利用 我们在2020年10月的成立会议期间收到的参与和热情的反馈，是 完全在线。这一新的杂交会议将集中在细胞、结构和生化机制上。 蛋白质毒素和效应器作用以及这些蛋白质的翻译用于治疗人类疾病。这个 会议将由西北大学主席Karla Satchell和副主席Steven Blanke组织 在伊利诺伊大学香槟分校学习。由十名成员组成的科学顾问委员会将协助遴选 演讲者和摘要回顾。将有5次会议和3次主旨演讲(罗德·韦尔奇博士来自 威斯康星大学；德克萨斯大学休斯顿分校的Theresa Koehler博士和耶鲁大学的Craig Roy博士 大学)。另外10位受邀演讲者将主持五个科学会议，这些会议将以演讲为主题。 从摘要中挑选。在这里，我们正在寻求NIAID资金1)来支持主旨演讲的注册费和旅费 并邀请发言者，(2)为代表不足的少数族裔博士后提供注册奖学金，以及 以及(3)为将同时现场直播的混合会议提供视听支持。 NIAID的这项支持将把微生物学家、生物化学家、结构生物学家和生物物理学家聚集在一起 鼓励跨越疾病和系统发育边界的新合作，并测试创新模式 这次会议的未来是一个混合型会议，将影响到全球观众。

PROJECT SUMMARY/ABSTRACT Protein toxins and effectors are primary virulence factors of many bacterial pathogens. They cause diseases by targeting and disrupting key cellular functions. Historically, research on toxins/effectors has focused on only a few major toxins, which has achieved tremendous successes in establishing the framework of their mechanisms and in eradicating many devastating infectious diseases. Building on these earlier successes, the field has gone through a great resurgence and expansion in the past 20 years, transforming from a narrow focus on a few human diseases to much broader areas of biology, centering on understanding “protein-based” fight-and- defense communications between co-evolving organisms. For instance, recent advances in microbial genomics and discovery-based screening approaches have led to a dramatic increase in the number of toxins and effector systems being studied. New research has also revealed that microbes secrete and inject toxins and effectors not only to target eukaryotic cells during pathogenesis, but also to engage in interbacterial competition. Technological advances in high-resolution microscopy, structural biology, cryo-electron microscopy, proteomics, and CRISPR have rapidly advanced our understanding of toxins/effectors action. Despite the growth of both the depth and breadth of the field, there is currently no meeting in North America centered on toxins/effectors that can bring people together from diverse fields. To address this gap, we are launching a reconfigured and reimagined “in person-virtual” hybrid conference, “Lakeside Conference on Protein Toxins and Effectors” (#LakesidePTE) to be held October 3-6, 2021 at the Abbey Resort in Lake Geneva, Wisconsin, USA, leveraging the participation and enthusiastic feedback received during our inaugural meeting in October 2020, which was entirely on-line. This newly hybrid conference will focus on the cellular, structural, and biochemical mechanisms of protein toxin and effector action and the translation of these proteins for treatment of human disease. The conference will be organized by Chair Karla Satchell at Northwestern University and Vice-Chair Steven Blanke at University of Illinois at Urbana-Champaign. A ten member scientific advisory board will assist in selection of speakers and abstract review. There will be 5 sessions and three keynote addresses (Dr. Rod Welch from University of Wisconsin; Dr. Theresa Koehler from University of Texas-Houston, and Dr. Craig Roy from Yale University). Ten additional invited speakers will anchor five scientific sessions that will be filled out with talks selected from abstracts. Here we are seeking NIAID funds 1) to support registration fee and travel for keynote and invited speakers, (2) to offer registration scholarship awards for underrepresented minority post-docs and graduate students and (3) for audiovisual support for a hybrid meeting that will be simultaneously live streamed. This support from NIAID will bring together microbiologists, biochemists, structural biologists, and biophysicists to encourage new collaborations across disease and phylogenetic boundaries and test an innovative model for the future of this conference as a hybrid conference that will reach a global audience.