Shared Resource: Cellular Imaging

项目摘要：蜂窝成像共享资源(CISR) 蜂窝成像共享资源(CISR)为各种高级成像和 探索细胞和模式生物结构和功能的定量图像分析技术 从分子层面到细胞层面。支持的应用包括扫描和传输电子 显微镜、电子显微镜(EM)样品制备、透射光和荧光显微镜 模式(包括共聚焦、去卷积、全内反射荧光显微镜(TIRF)、超 分辨率、延时、整张幻灯片扫描和高内容筛选)，以及基于实时图像的蜂窝 最近实施的检测方法支持不断增长的癌症免疫学研究计划。其他内容 服务包括定量凝胶和印迹分析以及核型分析。国际疾病控制与预防中心由Dr。 胡里奥·巴斯克斯，由两名工作人员(1.65 FTE)和一名外部顾问协助。 2015-2018年期间，《销售公约》从大约150个用户向500多个个人用户提供了服务 代表该联盟所有研究计划的研究小组。仅在2018财年，《公约》就支持 超过200项研究拨款。CSR向该联盟引入了许多新技术，支持和 通过提供尖端仪器和专业知识加速癌症研究，培训新用户了解 每项技术，并在所有支持的技术方面与研究人员密切合作。虽然许多实验室都有 除了自己用于日常工作的仪器外，许多先进技术只有在《公约》中才能获得。 1

PROJECT SUMMARY: CELLULAR IMAGING SHARED RESOURCE (CISR) The Cellular Imaging Shared Resource (CISR) provides support with a broad range of advanced imaging and quantitative image analysis technologies for probing the structure and function of cells and model organisms from the molecular to the cellular level. Supported applications include scanning and transmission electron microscopy, electron microscopy (EM) sample preparation, transmitted light and fluorescence microscopy modalities (including confocal, deconvolution, total internal reflection fluorescence microscopy (TIRF), super- resolution, time-lapse, whole slide scanning, and high-content screening), and live image-based cellular assays recently implemented to support the growing Cancer Immunology Research Program. Additional services include quantitative gel and blot analysis as well as karyotyping. CISR is under the leadership of Dr. Julio Vazquez who is assisted by two staff members (1.65 FTE) and one external consultant. For the period 2015-2018, CISR provided services to more than 500 individual users from approximately 150 research groups representing all of the Consortium’s Research Programs. In FY 2018 alone, CISR supported over 200 research grants. CISR has introduced many new technologies to the Consortium, supports and accelerates cancer research by providing leading edge instrumentation and expertise, training users new to each technique, and working closely with researchers in all supported technologies. While many labs have their own instruments for routine work, many advanced technologies are only available in the CISR. 1