Core A: Mouse Core

STAT3变异体作为免疫耐受小鼠核心的变阻器 项目摘要 转基因小鼠的使用彻底改变了对复杂生物过程的研究，包括 免疫耐受和自身免疫。本P01中的所有项目都严重依赖转基因技术的使用 转基因、基因敲除和有自身免疫倾向的小鼠品系。许多自体免疫倾向和免疫缺陷 LINE需要特定的专业知识和条件才能进行最佳研究，包括在无病原体的环境中进行研究 环境。此外，这种动物的饲养和照料在处理 多个转基因品系和不同遗传品系的小鼠。人们设想了一款鼠标内核，它将 通过生成和维护基因复杂的品系来帮助简化和提高效率 一个健康、无病原体的环境。因为几个P01项目对点点鼠标的依赖 糖尿病模型和新的STAT3功能获得(GOF)小鼠系，许多核心努力将 围绕着这些线路的维护、分配和使用，结合其他相关免疫 系统记者或免疫缺陷线路。通过这种方式，核心可以帮助确保质量，遗传血统， 以及共享P01调查人员合作研究所需的动物。

STAT3 Variants as a Rheostat of Immune Tolerance Mouse Core Project Summary The use of genetically altered mice has revolutionized the study of complex biological processes, including immune tolerance and autoimmunity. All the projects in this P01 rely heavily on the use of genetically modified transgenic, knockout, and autoimmune-prone mouse strains. Many autoimmune prone and immunodeficient lines require specific expertise and conditions for optimal research, including housing in pathogen-free environments. Additionally, breeding and care of such animals can become complex when dealing with multiple genetically modified lines and different genetic strains of mice. A mouse Core is envisioned that will help streamline and improve efficiencies with the generation and maintenance of genetically complex lines in a healthy, pathogen-free environment. Because of the reliance of several P01 projects on the NOD mouse model of diabetes and the novel STAT3 gain of function (GOF) mouse lines, much of the Core efforts will revolve around the maintenance, distribution and use of these lines in combination with other relevant immune system reporters or immunodeficient lines. In this way, the Core can help ensure the quality, genetic lineage, and sharing of animals needed for the collaborative studies of the P01 investigators.