Optical Molecular Imaging Core

摘要-光学分子成像与分析共享资源 光学分子成像和分析(OMIA)共享资源提供体内光学成像和 光谱范围从整个动物的荧光和生物发光到功能活体显微镜。 OMIA服务大致可按以下类别细分：1)光学成像，2)窗口 腔内手术培训和支持，3)用户技术培训，4)实验科学指导 设计、数据分析和解释。 在光学成像中，两种主要的技术方法是活体整体动物生物发光和 荧光成像。这些是使用一套商业成像设备实现的，即IVIS Lumina 和Kinetic，以及FMT2500LX荧光断层扫描系统(均来自PerkinElmer)。第三个专业 光学成像的技术方法是活体显微镜。此方法通过以下方式促进 多年来在共享资源联合主任的实验室中开发的资源。可用的 资源包括手术设施和进行手术所需的用品，以及显微镜。 适用于活体功能成像。这三种光学成像功能加在一起， OMIA的专业知识、培训和服务为DCI成员研究癌症提供了独特的资源 治疗学和肿瘤生物学。 这是一项至关重要的共享资源，2018年为38名调查人员提供了服务，其中71%是DCI 成员，占总使用量的74%，来自所有8个DCI研究项目。DCI成员受益于 特别是由于可获得与DCI贡献成比例的成本补偿(通常为10%-30% 基于服务)，以及广泛的癌症模型，这些模型占使用的大部分 这些服务中。这一机制对DCI成员的影响最好地突出了这一事实，即 在项目所涉期间，资源为83份出版物作出了贡献，其中15份刊登在影响较大的期刊(Impact 要素&gt；9)，表明资源提供的服务的价值。

ABSTRACT – OPTICAL MOLECULAR IMAGING AND ANALYSIS SHARED RESOURCE The Optical Molecular Imaging and Analysis (OMIA) shared resource provides in vivo optical imaging and spectroscopy ranging from whole animal fluorescence and bioluminescence to functional intravital microscopy. OMIA services can be broken down broadly according to the following categories: 1) optical imaging, 2) window chamber surgery training and support, 3) user training in techniques, and 4) scientific guidance on experimental design, data analysis and interpretation. Within optical imaging, two primary technological approaches are in vivo whole animal bioluminescence and fluorescence imaging. These are achieved using a set of commercial imaging devices, namely the IVIS Lumina and Kinetic, as well as FMT2500LX fluorescence tomography system (all from PerkinElmer). A third major technological approach within optical imaging is intravital microscopy. This approach is facilitated through resources developed over many years in the laboratories of the shared resource co-directors. The available resources include a surgical facility and the supplies needed for performing surgeries, as well as microscopes suitable for intravital functional imaging. Together, these three optical imaging capabilities, plus the other expertise, training, and services of OMIA, provide a unique resource for DCI members to study cancer therapeutics and tumor biology. This is a vital shared resource that, in 2018, provided services to 38 investigators, 71% of whom were DCI members, accounting for 74% of total usage, from all 8 DCI Research Programs. DCI members benefit in particular due to the availability of cost offsets that are proportional to the DCI contribution (typically 10-30% based on the service), as well as a wide range of cancer models, which account for the majority of the utilization of these services. The impact of this facility on DCI members is best highlighted by the fact that this shared resource contributed to 83 publications over the project period, 15 of which were in high impact journals (Impact Factor >9), demonstrating the value of services offered by the resource.