Cell Biology Core

项目摘要-细胞生物学核心 自1977年成立以来，细胞生物学一直是DRTC的一个组成部分。它有很多种形式 在过去的40年里，最近专注于胰岛和β细胞的研究。然而， 在过去的18个月中，在这一应用中，细胞生物学核心已经扩大到也包括 正常人和糖尿病患者脂肪细胞、肝细胞、先天性免疫细胞和获得性免疫细胞的研究 人类和老鼠。核心在基于蜂窝的系统中提供了广泛的服务，以了解 胰岛内分泌细胞、脂肪细胞、肝细胞和免疫细胞在健康和疾病中的作用 使用人类和小鼠的原代细胞进行研究的重点。核心提供学习方面的建议 设计、方法和数据解释。由于许多技术对调查人员来说都是新的，它还 提供直接的实际支持和培训，以确保成功和及时完成 实验。该中心位于芝加哥大学和伊利诺伊大学芝加哥分校。 这两个小组定期会面，并制定了标准的运营周期，以确保用户 为他们的学习提供优质的支持。核心还持续致力于培训，并已 对研究人员、研究员、学生和研究技术人员进行核心程序方面的培训。《细胞》 生物核心是DRTC不可分割的一部分，因此与昼夜生物学和 生理学核心，通过促进基于糖尿病的小鼠原代细胞和组织研究 人类。此外，它还允许对遗传学和基因组学核心中的发现进行测试 生物学背景。

PROJECT SUMMARY – CELL BIOLOGY CORE Cell Biology has been a component of the DRTC since its inception in 1977. It has taken many forms over the past 40 years, most recently focusing on studies of pancreatic islets and beta cells. However, in the past 18 months and in this application the Cell Biology Core has been broadened to also include studies of adipocytes, hepatocytes, and innate and adaptive immune cells from normal and diabetic humans and mice. The Core provides a broad range of services in cell-based systems to understand the roles of islet endocrine cells, adipocytes, hepatocytes and immune cells in health and disease with an emphasis of studies using primary cells from humans and mice. The Core provides advice on study design, methods and data interpretation. Since many of the techniques are new to investigators, it also provides direct hands-on support and training to ensure the successful and timely completion of experiments. This Core is centered at the University of Chicago and the University of Illinois at Chicago. The two groups meet regularly and have developed standard operating periods to ensure that Users receive quality support for their studies. The Core also has an ongoing commitment to training and has trained investigators, fellows, students and research technicians in the procedures of the Core. The Cell Biology Core is an integral part of the DRTC, and thus synergizes with the Circadian Biology and Physiology Cores, by facilitating diabetes based research on primary cells and tissues from mice and humans. Moreover, it allows discoveries made in the Genetics and Genomics Core to be tested in a biological context.