Linear Ion Trap - Quadrupole LC-MS System

项目摘要/摘要 这项建议是为了建立一个新的灵敏和更宽质量范围的LC/MS系统，这两个系统都可以 增强我们的Lipidology核心设施的现有能力并提供备份 为NIH资助的韦恩州立大学的研究人员提供老化的质谱学资源 大学。目前，韦恩州立大学的质谱学资源 在卢米根化学仪器中心，药理学的核心 卡马诺斯癌症研究所(KCI)、蛋白质组学核心和脂质组学核心。 然而，现有的工具都不够灵敏，无法准确量化 脂类调节剂，如专业的支持分解调节剂(SPM)， 在皮摩尔浓度下具有生理活性。对此类生物分子的有限分析 从患者身上采集的样本量要求在低血像中具有敏感度 射程。参与研究的教员的主要领域之一涉及识别 和/或定量这种炎症和炎症消退的脂质介质 (例如，SPM)在癌症、糖尿病、心血管疾病和神经退行性疾病中 精神错乱。脂肪组学核心设备目前使用已有10年历史的LC-MS系统， 有限的质量范围(&lt；1200 Da)，不足以分析脂体的重要部分， 而灵敏度比分析重要脂质所需的灵敏度低约10倍 调解人。线性离子陷阱三重四极质量的建议获取 光谱分析系统以其高灵敏度、快速扫描、5阶以上 超高压液体的量值动态范围 色谱系统填补了我们在分析需求方面的一个巨大空白。鉴于这一事件的焦点 用户对新的炎症和相关小分子生物标志物的识别 慢性疾病，如癌症和糖尿病，这台质谱仪是紧急和至关重要的 用于我们的研究项目。此外，这一工具将催化我们的 将脂肪组学核心设施转化为韦恩州立大学成熟的新陈代谢组学核心。

Project Summary/Abstract This proposal is for a new sensitive and wider mass range LC/MS system, both to enhance the existing capabilities of our Lipidomics Core Facility and to provide a backup for the aging mass spectrometry resource to NIH funded researchers at Wayne State University. Currently mass spectrometry resources at Wayne State University are located at the Lumigen Instrumentation Center in Chemistry, the Pharmacology Core of the Karmanos Cancer Institute (KCI), the Proteomics Core, and the Lipidomics Core. However, none of the available instruments are sensitive enough to accurately quantify lipid mediators such as Specialized Pro-resolution Mediators (SPMs) that are physiologically active at picomolar concentrations. Analyzing such biomolecules in limited sample volumes that are collected from patients require sensitivity in the low femtogram range. One of the major areas of the participating faculty's research involves identification and/or quantitation of such lipid mediators of inflammation and resolution of inflammation (e.g. SPMs) in diseases such as cancer, diabetes, cardiovascular and neurodegenerative disorders. Lipidomics Core Facility currently utilizes a 10-year-old LC-MS system with limited mass range (<1200 Da), insufficient to analyze a significant part of the lipidome, and sensitivity that is about 10-times lower than required for the analysis of important lipid mediators. The proposed acquisition of a linear ion-trap triple quadrupole mass spectrometry system with its high sensitivity, fast scanning, more than 5 orders of magnitude dynamic range of quantitation along with an ultra-high pressure liquid chromatographic system fills a great void in our analytical needs. Given the focus of the users on the identification of novel small molecule biomarkers of inflammation and related chronic diseases such as cancer and diabetes, this mass spectrometer is urgent and vital for our research projects. Additionally, this instrument will catalyze the evolution of our Lipidomics core facility into a full-fledged metabolomics core at Wayne State University.