NovaSeq 6000 Sequencing System

标题：NovaSeq 6000高通量DNA测序仪 项目概要/摘要。 UAB基因组学核心正在申请资金购买Illumina NovaSeq 6000 NextGeneration DNA测序仪将测序需求的增加与数据输出增加的预期结合起来 低成本的核心的用户与适当的技术。NovaSeq 6000（NovaSeq）目前是 市场上最高通量的测序仪器，可产生高达6万亿个碱基的序列 从一台仪器运行。NovaSeq对我们NIH资助的研究人员的优势是：1）获得 最先进的测序技术，2）执行核心电流禁止的实验的能力 仪器，3）显著的成本节约，4）在批次效应方面减少实验偏差，以及5） 减少数据采集时间。基因组学核心是UAB校园内唯一的基因组学核心 服务于医学院、研究生院、本科校园，是综合性的 奥尼尔综合癌症中心结构内的基因组学共享资源。核心目前 在NextSeq 500和MiSeq上处理用于各种高通量测序测定的样品。 多个UAB研究者对测序的需求增加， 增加的测序深度使我们的NextSeq系统几乎过时。自20年前成立以来， Genomics Core致力于为遗传和基因组学提供先进的技术， research.核心已经支持了来自校园的188名教师，其中86名获得了NIH奖项。此外，核心 支持了邻近机构的各种调查人员，如南亚拉巴马大学， 亚拉巴马州立大学和埃默里大学。核心支持的研究是令人难以置信的多样化 包括癌症生物学，肾脏学，免疫学，癌症免疫学，生态学，心脏发育， 纤毛病变，骨骼发育，神经生物学，细菌-宿主相互作用，宏基因组学，SARS-CoV-2 序列分析、表观遗传学和单细胞测序分析。将NovaSeq添加到UAB Genomics Core将使我们能够为研究人员提供他们所需的测序能力， 这是他们预期的价格点，并将允许核心扩展服务。

Title: NovaSeq6000 High-Throughput DNA Sequencer Project Summary/Abstract. The UAB Genomics Core is requesting funds to purchase an Illumina NovaSeq6000 NextGeneration DNA sequencer to align the increased demand for sequencing and expectations of increased data output at low cost of the core’s users with the appropriate technology. The NovaSeq6000 (NovaSeq) is currently the highest throughput sequencing instrument on the market and can produce up to 6 trillion bases of sequence from one instrument run. The advantages of the NovaSeq to our NIH funded investigators are 1) access to state-of-the-art sequencing technology, 2) ability to perform experiments prohibited with the core’s current instruments, 3) significant cost savings, 4) reduction in experimental bias in terms of batch effect, and 5) decreased times for data acquisition. The Genomics Core is the only genomics core on the UAB campus and serves the medical school, graduate school, undergraduate campus, and is the Comprehensive Genomics Shared Resource within the O’Neal Comprehensive Cancer Center structure. The core currently processes samples for various high throughput sequencing assays on a NextSeq500 and a MiSeq. Increased demand for sequencing from multiple UAB investigators combined with the requirement of increased sequencing depth make our NextSeq system near obsolete. Since its inception 20 years ago, the Genomics Core has dedicated its resources to providing advanced technologies for genetic and genomic research. The core has supported 188 faculty from across campus, 86 with NIH awards. Moreover, the core has supported various investigators at neighboring institutions such as University of South Alabama, Alabama State University, and Emory University. The research supported by the core is incredibly diverse and includes cancer biology, nephrology, immunology, cancer immunology, ecology, cardiac development, ciliopathies, skeletal development, neurobiology, bacterial-host interactions, metagenomics, SARS-CoV-2 sequence analysis, epigenetics and single cell sequencing analysis. The addition of the NovaSeq to the UAB Genomics Core will allow us the ability to provide investigators with the sequencing capacity they demand at a price point they expect and will allow the core to expand services.