Levelling up: Cultured meat bioprocess intensification for scalable commercial production

养殖肉类为解决传统肉类行业的可持续性、动物福利和环境影响问题提供了巨大的潜力。然而，在扩大生产以满足市场需求方面仍然存在技术经济挑战，预计到2030年将达到200亿美元。这种生产方法消除了饲养和农场动物的需要，这意味着养殖肉类生产对环境的影响比传统的同类产品要低得多。培养肉由相同的细胞类型组成，这些细胞类型可以排列成与动物组织相同的结构，以复制传统肉的味道，质地和营养特征。由于大多数细胞系的寿命及其分化成所需细胞类型的能力，Roslin Technologies（RTL）是第一家为养殖肉类行业提供高质量细胞系的领先供应商。我们开发的诱导多能干细胞系（iPSC）可以分化成肌肉和脂肪，由定制的细胞培养基和工艺支持。我们的细胞非常适合培养肉类行业，因为它们是非转基因的，生长迅速，遗传稳定，可以在悬浮培养中生长，而无需额外的支架。在RTL，我们已经证明了我们的细胞可以在小型台式生物反应器中生长，但下一步是将其转移到行业相关的中试规模生物反应器中。在Innovate UK和Innosuisse的支持下，RTL很高兴与瑞士Kempttthal的The Cultured Hub（TCH）合作。TCH是一家专门的养殖肉类合同开发和制造组织（CDMO），提供工艺强化和规模扩大方面的专业知识。在这个项目中，RTL和TCH将共同努力实现RTL干细胞的全部潜力。研究人员将通过培养基和生物工艺开发来优化细胞产量、质量和安全性。该项目产生的数据和技术将有助于证明RTL细胞的商业潜力，并将TCH定位为培养肉生物工艺开发的领先CDMO。该项目侧重于养殖肉类的安全性，也将为监管机构和政策制定者提供有价值的信息，以帮助确定英国的CM质量和安全标准。我们相信这将有利于养殖肉类生产商和整个行业更快地进入大众消费市场。最终，这将支持一个更具弹性的粮食系统，并有助于实现《巴黎协定》目标和《联合国气候变化框架公约》。

Cultured Meat offers enormous potential for addressing concerns around sustainability, animal welfare and environmental impact of the traditional meat industry. However, technoeconomic challenges remain in scaling production to meet market demand, estimated to reach $20Bn by 2030\.Cultured meat is produced by directly culturing animal cells. This production method eliminates the need to raise and farm animals for food, meaning that cultured meat production has a considerably lower environmental impact than its conventional counterpart. Cultured meat consists of the same cell types that can be arranged in the same structure as animal tissues to replicate the taste, texture and nutritional profiles of conventional meat.Producing cultured meat can be challenging due to longevity of most cell lines and their abilities to differentiate into required cell types, including muscle and fat.Roslin Technologies (RTL) are the first and leading provider of high quality cell lines for the cultivated meat sector. We develop induced pluripotent stem cell lines (iPSC) that can differentiate into muscle and fat, supported by customised cell culture media and processes. Our cells are well suited to the cultured meat industry as they are non-GMO, grow quickly, are genetically stable and can grow in suspension culture without the need for additional scaffolds. At RTL we have demonstrated our cells can grow in small-scale benchtop bioreactors, but the next step is to transfer this to industry relevant pilot-scale bioreactors.With Innovate UK and Innosuisse support RTL are excited to partner with The Cultured Hub (TCH) in Kemptthal, Switzerland. TCH is a dedicated cultured meat contract development and manufacturing organisation (CDMO) that provides expertise in process intensification and scale-up. In this project RTL and TCH will work together to realise the full potential of RTL stem cells. Researchers will work to optimise cell yields, quality and safety through media and bioprocess development. The data and know how generated by this project will help demonstrate the commercial potential of RTL cells and position TCH as a leading CDMO for cultured meat bioprocess development. The projects focus on cultured meat safety will also provide valuable information to regulators and policy makers to help define UK CM quality and safety standards. We believe this will benefit cultured meat producers and the industry as a whole to reach mass consumer markets faster. Ultimately this will support a more resilient food system and contribute to Paris Agreement goals and the UN Framework Convention on Climate Change.