Voltage clamp elecrophysiological set-up for cultured neurons

培养神经元的电压钳电生理学设置

• 批准号: 376123-2009
• 负责人: Spencer, Gaynor
• 金额: $ 2.86万
• 依托单位: Brock University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412309
• 关键词: Voltage; clamp; elecrophysiological; set; up

My research program involves the study of regeneration of cells of the central nervous system, as well as cellular mechanisms underlying learning and memory. The animal model I use in these studies is the mollusc, Lymnaea stagnalis, as its brain cells are large, easily accessible and many are individually identified with known transmitters (e.g. dopamine). Many signaling pathways and neuronal mechanisms are conserved between vertebrates and invertebrates, so research findings from this model may directly influence our knowledge of higher nervous systems. The research I am conducting includes recordings from brain cells in a cultured environment, as well as from intact animals and isolated nervous systems. I currently have three recording set-ups in my laboratory, but only one of these is configured for recordings from cultured cells. My research program has been steadily progressing in the area of cultured neurons, and I now need to convert one of my recording set-ups for use with cultured cells.

我的研究项目涉及中枢神经系统细胞再生以及学习和记忆的细胞机制的研究。我在这些研究中使用的动物模型是软体动物 Lymnaea stagnalis，因为它的脑细胞很大，易于接近，并且许多细胞都用已知的递质（例如多巴胺）单独识别。许多信号通路和神经机制在脊椎动物和无脊椎动物之间是保守的，因此该模型的研究结果可能会直接影响我们对高级神经系统的认识。我正在进行的研究包括培养环境中脑细胞的记录，以及完整动物和分离神经系统的记录。我的实验室目前有三套记录装置，但其中只有一套配置用于从培养细胞进行记录。我的研究项目在培养神经元领域一直在稳步进展，现在我需要将我的记录装置之一转换为用于培养细胞。