Detection and surveillance of antimicrobial resistance among enteric bacteria from raw retail meat and seafood in Iowa: A NARMS Retail Food Surveillance Project

项目摘要/摘要 生源肠道细菌耐药性的检测与监测 爱荷华州肉类和海鲜零售业：NARMS零售食品监测项目 抗菌素耐药性(AMR)是影响人类福祉的主要健康威胁 世界各地的人类、动物和环境。强大而全面的监控 强调“一个健康”方法的系统对于有效地打击AMR是必要的。 在美国，国家抗菌素耐药性监测系统的零售部门 (NARMS)隶属于食品和药物管理局兽医中心(FDA- CVM)对选定的肠道细菌(非伤寒沙门氏菌、 来自零售生肉(鸡肉，磨碎的鸡肉)的弯曲杆菌、肠球菌和大肠杆菌) 火鸡和牛肉，以及碎/碎猪肉)在18个州。最近，零售中的AMR监测 海鲜(罗非鱼、虾、三文鱼)对选定的细菌(弧菌、气单胞菌、大肠杆菌、肠球菌、 生产碳青霉烯酶的肠杆菌)也已在至少8个州启动。这个 NARMS计划在监测AMR的来源和趋势方面做出了重大贡献 食物链。为了加强抗击AMR的努力，FDA发起了一个合作社 2016年达成协议，将NARMS监视网络扩大到全美。 在这一倡议下，爱荷华州立大学(ISU)于晚些时候成为NARMS网络实验室 2016年。自那时以来，执行支助股网站大大促进了加强能力和 零售食品中国家AMR监测项目的有效性。在此应用程序中，我们 建议继续与NARMS建立协作伙伴关系，以实现以下最新目标 FOA PAR-20-124《NARMS合作社》中所述的零售食品监管计划 加强零售食品抗生素耐药性监测的协议计划 标本“。具体来说，我们会继续收集和处理生肉和海鲜样本。 从爱荷华州的杂货店采集样本并进行培养，以分离出感兴趣的细菌 由NARMS定义，并每月将分离株运往FDA。此外，我们还将 继续进行沙门氏菌、弯曲杆菌和大肠杆菌的全基因组测序 从肉类样本中分离并通过GenomeTrakr与FDA共享序列。这 应用程序建立在现有经验的基础上，是我们当前工作的自然延伸 纳尔马斯。需要训练有素的人员和后勤基础设施 计划中的工作已经到位。我们坚信，执行支助股将继续参与这项工作 协作网络将大大有助于减少食物链中的AMR和 最终促进食品安全和公众健康。

Project Summary/Abstract Detection and surveillance of antimicrobial resistance among enteric bacteria from raw retail meat and seafood in Iowa: A NARMS Retail Food Surveillance Project Antimicrobial resistance (AMR) is a major health threat affecting the well-being of humans, animals, and the environment worldwide. Robust and comprehensive surveillance systems emphasizing the “One Health” approach are necessary for effectively combatting AMR. In the United States, the retail arm of the National Antimicrobial Resistance Monitoring System (NARMS) under the Food and Drug Administration, Center for Veterinary Medicine (FDA- CVM) performs surveillance of AMR in select enteric bacteria (non-typhoidal Salmonella, Campylobacter, Enterococcus and Escherichia coli) from retail raw meats (chicken, ground turkey and beef, and ground/chop pork) in 18 states. Recently, surveillance of AMR in retail seafood (tilapia, shrimp, salmon) for select bacteria (Vibrio, Aeromonas, E. coli, Enterococcus, carbapenemase-producing Enterobacteriaciae) has also been started in at least 8 states. The NARMS program has contributed significantly to monitoring the sources and trends of AMR in the food chain. To enhance the effort on combating AMR, FDA launched a cooperative agreement program to expand the NARMS surveillance network throughout the U.S in 2016. Under the initiative, Iowa State University (ISU) became a NARMS network laboratory in late 2016. Since then, the ISU site has contributed substantially to strengthening the capacity and effectiveness of the national AMR surveillance program in retail food. In this application, we propose to continue the collaborative partnership with NARMS to address the updated goals of the Retail Food Surveillance Program as described in FOA PAR-20-124 “NARMS Cooperative Agreement Program to Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens”. Specifically, we will continue to collect and process raw meat samples and seafood samples from grocery stores in Iowa and culture them for isolation of bacteria of interest as defined by NARMS, and ship the isolates to FDA on a monthly basis. In addition, we will continue to perform whole-genome sequencing of Salmonella, Campylobacter and E. coli isolates from the meat samples and share the sequences with FDA via GenomeTrakr. This application is built on existing experience and is a natural extension of our current effort with NARMS. Trained personnel and logistic infrastructure required for successful completion of the planned work are already in place. We strongly believe that ISU’s continued participation in this collaborative network will contribute significantly to mitigation of AMR in the food chain and ultimately to enhancement of food safety and public health.