Microbial Genomics and Analytical Laboratory (M-GAL)

项目概要-核心C：微生物基因组学和分析实验室（M-GAL） 微生物基因组学和分析实验室（M-GAL）环境综合中心的核心 微生物组和人类健康将提供一个集中的设施和技术专长在大学 Hawai`i at Mānoa（UHM）支持微生物环境与人类之间界面的研究 健康通过下一代测序和分析表征微生物群落组成 微生物代谢产物的定量分析是微生物组研究领域中使用的标准方法。使 这些应用程序，M-GAL核心将提供以下内容：（一）设备和专门知识的准备 基因组文库和基因表达的定量;（ii）高效液相色谱法和 用于分析脂质和代谢物的质谱仪器;（iii）用于培养的设施， 微生物菌株的分离和长期储存;以及（iv）社区可搜索数据库的管理 包含有关从夏威夷本土动物群和生态系统中分离出的菌株的信息。M-GAL核心 将提供给整个夏威夷州的研究人员和来访的科学家，从而鼓励更多的 当地微生物组研究界之间的互动和国外的合作努力。 M-GAL核心将通过提供必要的信息， 基础设施和专业知识，以推进他们的研究和促进初级教师的职业生涯。而且 核心将支持UHM作为太平洋地区微生物组研究的主要中心的发展， 提供文库制备和代谢物分析方面的实践培训，举办国际暑期 学校的基本微生物组研究方法，并促进外联工作，以特点 夏威夷的微生物 !

Project Summary – CORE C: Microbial Genomics and Analytical Laboratory (M-GAL) The Microbial Genomics and Analytical Laboratory (M-GAL) Core of the Integrative Center for Environmental Microbiomes and Human Health will provide a centralized facility and technical expertise at the University of Hawai`i at Mānoa (UHM) to support the study of the interface between the microbial environment and human health. The characterization of microbial community composition by next-generation sequencing and analysis of microbial metabolic products are standard methods used in the field of microbiome research. To enable these applications, the M-GAL Core will provide the following: (i) equipment and expertise for the preparation of genomic libraries and the quantitation of gene expression; (ii) high-performance liquid chromatography and mass spectrometry instrumentation for the analysis of lipids and metabolites; (iii) facilities for the cultivation, isolation, and long-term storage of microbial strains; and (iv) curation of a community searchable database containing information about strains isolated from native Hawaiian fauna and ecosystems. The M-GAL core will be available to researchers throughout the state of Hawai'i and visiting scientists, thus encouraging greater interaction amongst the local microbiome research community and seeding collaborative efforts from abroad. The M-GAL Core will beneficially impact COBRE investigators and affiliates by providing essential infrastructure and expertise to advance their research and foster the careers of junior faculty. Moreover, the Core will support the growth of UHM as a major center for microbiome studies in the Pacific Region by providing hands-on training in library preparation and metabolite analysis, hosting an international summer school on fundamental microbiome research methods, and facilitating outreach efforts to characterize Hawai'i's microbiome. !