Vascularization in bone tissue engineering constructs

摘要 节段性大骨缺损的治疗仍然是一个重要的临床问题。Pre- 可与宿主血管床快速吻合的带血管大段骨移植 与天然骨组织结合将极大地改进目前可用的治疗方法。 解决这些问题有望克服组织的绊脚石之一 工程化和革命性的大型骨缺损的治疗。在这个项目中，我们建议 设计一种新型的预血管化骨移植替代物来修复大的骨缺损。这部小说 骨移植是由可生物降解的磷酸钙聚合物复合体组成的。 含有细胞水凝胶(作为骨替代物)和可弯曲的、可缝合的、 抗血栓、透性聚合物为基础的组织工程血管移植物(TEVG，作为血液 船只代管)。TEVG将以空间受控的方式整合到脚手架中 时尚。我们的假设是TEVG将促进和促进 支架，同时允许外科吻合术以使 工程化骨移植和宿主组织修复大型骨缺损。为了检验我们的假设，我们的 团队由生物材料、骨组织工程、新生血管、微血管、 外科和矫形外科提出以下目标：目标1是开发一种TEVG， 促进宿主血管的整合。目标2是设计和开发一种即时集成的、预 带血管的骨移植替代物。目标3是确定血管化骨的效率。 组织构建原型，加强与宿主骨组织的整合，加速骨骼 再生和恢复功能。这个项目的完成将带来革命性的变化 血管化人工骨移植设计，为改进临床治疗铺平道路 较大的节段性骨缺损。 好了！

Abstract Treatment of segmental large bone defects remains a significant clinical problem. Pre- vascularized large bone grafts capable of rapidly anastomosing with host vessel bed and integrating with native bone tissue would significantly advance currently available treatments. Addressing these issues is expected to overcome one of the stumbling blocks of tissue engineering and revolutionize the treatment of large bone defects. In this project, we propose to engineer a novel pre-vascularized bone graft substitute to repair large bone defects. The novel bone graft is comprised of a channeled, biodegradable calcium phosphate-polymer composite scaffold containing a cell-laden hydrogel (as a bone surrogate) and a flexible, suturable, antithrombotic, permeable polymer-based tissue engineered vessel graft (TEVG, as a blood vessel surrogate). The TEVG will be incorporated into the scaffold in a spatially controlled fashion. Our hypothesis is that the TEVG will facilitate and promote microvascularization across the scaffold, while allowing for surgical anastomosis to enable instant integration between the engineered bone graft and host tissue to repair large bone defects. To test our hypothesis, our team comprised of experts in biomaterials, bone tissue engineering, neovascularization, micro- surgery and orthopedic surgery, proposes the following aims:!Aim 1 is to develop a TEVG that facilitates host vascular integration. Aim 2 is to design and develop an instantly integrated, pre- vascularized bone graft substitute. Aim 3 is to determine the efficiency of a vascularized bone tissue construct prototype to enhance integration with host bone tissue, accelerate bone regeneration and restore functionality. The accomplishment of this project will revolutionize vascularized synthetic bone graft design, and pave the way for improved clinical treatments for large segmental bone defects. !