Genomic and Molecular Biology (GMB)

项目概要/摘要：基因组学和分子生物学 基因组学和分子生物学（GMB）共享资源提供先进的基因组学技术， 服务和专业知识，以支持在诺里斯棉花癌症中心进行的生物医学研究 （NCCC）和达特茅斯学院。专线小巴作为一个收费的服务设施提供DNA测序和 RNA、微阵列技术、单细胞DNA/RNA测序、NanoString分析和次日桑格 测序服务。GMB还提供检测设计和主要数据分析的专业知识， 补充服务。改善专线小巴在过去几年中包括一个新的和改进的样本 跟踪和计费系统（RaDar），以及升级所使用的仪器和协议。额外 改进包括增加新人员的专业知识和单细胞测序服务，新的 Nanostring测定和小鼠SNP基因分型。专线小巴及其承诺的工作人员有一个合并130+年的 临床和研究相关的实验室经验。这种专业知识和经验促进专线小巴的能力， 操作最先进的仪器，实施尖端的工作流程，为NCCC研究人员提供 在这个快速发展的领域中获得必要的基因组学技术。这也是至关重要的专线小巴留下来 与市场价格相比具有竞争力，同时为用户提供超过外部服务提供商的附加价值。 强大的机构支持和绿色专线小巴领导确保其稳定性.绿色专线小巴领导工作勤奋招聘 通过参加内部和外部研讨会， 两会专线小巴内的人员通过会议保持与新技术发展同步 出席以及生物分子研究设施协会（ABRF）的成员。专线小巴服务 癌症中心的成员，更广泛的达特茅斯研究社区，以及来自外部机构的用户， 以80%的容量运行。来自所有4个NCCC项目的六十一（61）名NCCC成员使用了GMB 共享资源，其中60人是4个计划（CPS [6]，CBT [37]，ICI [14]和TEC [3]）的现任成员。 NCCC资助的会员使用占总使用量的31%（4，779个项目），我们要求19%的 来自CCSG支持的GMB总运营预算。优先考虑NCCC成员样品，其次是 达特茅斯的样本，然后是外面的样本。绿色专线小巴保持严格的质量控制程序， 样本进入和数据离开设施。定期对所有仪器进行适当的控制， 随时间跟踪运行度量以确保最佳性能。未来的计划包括实施新的 NCCC研究人员高度寻求的单细胞工作流程，蛋白质和抗原条形码，以及DNA和ATAC- seq工作流。正在进行谈判，以获得空间基因组技术，如Nanostring Digital Spatial Profiler，作为基于液滴的单细胞分析的补充。绿色专线小巴正在实施解决方案， CRISPR/Cas-9编辑的全基因组检测，包括GUIDE-seq和CIRCLE-seq方法。 绿色专线小巴致力于提供NCCC研究人员与最新的基因组学专业知识和技术.

Project Summary/Abstract: Genomics and Molecular Biology The Genomics and Molecular Biology (GMB) Shared Resource provides advanced genomics technologies, services, and expertise in support of biomedical research conducted at the Norris Cotton Cancer Center (NCCC) and Dartmouth College. GMB operates as a fee-for-service facility offering sequencing of DNA and RNA, microarray technologies, single-cell DNA/RNA sequencing, NanoString assays, and next-day Sanger sequencing services. GMB also provides expertise for assay design and primary data analysis as complementary services. Improvements to GMB in the past several years include a new and improved sample tracking and billing system (RaDar), as well as up-grades in instrumentation and protocols used. Additional improvements include added expertise of new personnel and services for single-cell sequencing, new Nanostring assays, and mouse SNP genotyping. GMB and its committed staff have a combined 130+ years of clinical- and research-related laboratory experience. This expertise and experience facilitate GMB’s ability to operate state-of-the-art instrumentation and implement cutting-edge workflows, providing NCCC researchers with access to essential genomics technologies in this fast-moving field. It is also essential for GMB to stay competitive with market rates while providing additional value to its users over external service providers. Strong institutional support and GMB leadership ensure its stability. GMB leadership works diligently to recruit new projects to the shared resource through participation in both internal and external seminars and conferences. Personnel within GMB stay abreast of new technology developments through conference attendance as well as membership in the Association of Biomolecular Research Facilities (ABRF). GMB serves Cancer Center members, the broader Dartmouth research community, and users from outside institutions, operating at ~80% capacity. Sixty-one (61) NCCC Members from all 4 NCCC Programs utilized the GMB Shared Resource, 60 of whom are current Members in 4 Programs (CPS [6], CBT [37], ICI [14], and TEC [3]). NCCC Funded Member Use represented 31% of Total Use (4,779 Projects), and we are requesting 19% of the GMB Total Operating Budget from CCSG support. Priority is given to NCCC member samples, followed by Dartmouth samples, and then outside samples. GMB maintains stringent quality control procedures over samples entering and data leaving the facility. Appropriate controls are run regularly on all instrumentation, and run metrics are tracked over time to ensure optimal performance. Future plans involve implementing new single-cell workflows highly sought by NCCC researchers, protein and antigen barcoding, and DNA and ATAC- seq workflows. Talks are underway to acquire spatial genomic technologies, such as the Nanostring Digital Spatial Profiler, as a complement to droplet-based single-cell profiling. GMB is implementing solutions for genome-wide detection of CRISPR/Cas-9 editing, including the GUIDE-seq and CIRCLE-seq methodologies. GMB is committed to providing NCCC researchers with the latest genomics expertise and technologies.