CNS-Met Metabolomics Core

神经系统疾病和中枢神经系统（CNS）损伤显示出明显的代谢缺陷， 转破坏生物能量平衡，氧化还原状态和神经递质生物合成所需的 维持、突触活性、神经元稳态和神经炎症。因此，CNS 代谢研究引起了那些研究阿尔茨海默病，创伤性脑损伤， 癫痫、脊髓损伤、帕金森氏病等。现代代谢组学允许 数百种代谢物。它既有信息性又有变革性， 假设生成和假设驱动研究。代谢组学目前的应用包括， 代谢疾病的分子基础，生物标志物发现，疾病预测，药物反应，以及 个性化医疗 CNS-Met代谢组学核心的首要目标是使尖端的代谢组学和 线粒体能量学技术和分析提供给CNS-Met研究项目负责人（RPL）， 试点项目PI。可用的专业知识和仪器与RPL建议保持一致。核心 董事们与每个RPL合作，以产生令人兴奋的初步数据。核心将参与，协助 实验设计、运行样本、培训人员和分析大脑代谢数据。CNS-Met Metabolomics Core将为CNS代谢领域的优秀RPL提供支持，并提高他们的成功率 竞争国家卫生研究院研究级赠款 为了实现这些目标，核心将目标1：提供实验设计咨询和项目特定 学习规划。核心小组与可重复使用许可证密切合作，并将继续这样做，在实验设计方面， 数据处理和生物统计分析。适当的实验设计和样品制备将奠定 代谢组学实验成功的基础目标2：提供代谢组学专业知识和培训。 核心将容纳Agilent 5977 B GCMS，2）Agilent 6545四极杆飞行时间LC/CE-QTOF，和3）Agilent 5977 B GCMS。 Seahorse Biosciences XF 24 e和XFe 96通量分析仪以及Oroboros O2 K荧光计。芯会 运行样本，协助，培训和/或监督数据分析，从基本数据简化，结合程序 控制、规范化、路径本体匹配、数据解释，并提供可能的后续步骤。目标3： 进行样品处理、技术分析和数据处理-解释-展示。芯会 协助用户进行样品制备、仪器操作、质谱峰匹配， 代谢物与已知化合物库、样品标准化和统计分析。 核心将利用核心领导层从实验设计中建立的综合流程， 快速一致的样品采集、样品固定、样品提取、数据简化、统计分析， 数据分析和生物学解释，以确保CNS-Met RPL和试点项目PI的成功。

Neurological disorders and central nervous system (CNS) injuries show clear metabolic defects, which in turn disrupts bioenergetics balances, redox status, and neurotransmitter biosynthesis that are required for maintaining, synaptic activity, neuronal homeostasis, and neuroinflammation. For these reasons, CNS metabolism research has generated interest from those studying Alzheimer’s disease, traumatic brain injury, epilepsy, spinal cord injury, Parkinson’s disease, and others. Modern metabolomics allows for the profiles of hundreds of metabolites from a single sample. It is both informative and transformative, supports both hypothesis-generating and hypothesis-driven research. Current applications of metabolomics include, molecular basis of metabolic diseases, biomarker discovery, disease prediction, drug responses, and personalized medicine. The overarching goal of the CNS-Met Metabolomics Core is to make cutting-edge metabolomics and mitochondrial energetics techniques and analyses available to CNS-Met Research Project Leaders (RPL) and pilot project PIs. The available expertise and instrumentation are well-aligned with the RPL proposals. The core directors have worked with each RPL to generate exciting preliminary data. The core will engage with, assist in experimental design, run samples, train personnel, and analyze brain metabolism data. The CNS-Met Metabolomics Core will support the talented RPLs in the area of CNS metabolism and enhance their success in competing for NIH R-level grants. To achieve these goals, the core will Aim 1: Provide experimental design consultation and project-specific study planning. The core has worked closely with the RPLs, and will continue to do so, on experimental design, data processing, and biostatistical analyses. Proper experimental design and sample preparation will lay the foundation for a successful metabolomics experiment. Aim 2: Provide metabolomics expertise and training. The core will house an Agilent 5977B GCMS, 2) Agilent 6545 quadrupole time of flight LC/CE-QTOF, and 3) a Seahorse Biosciences XF24e and XFe96 Flux Analyzers as well as Oroboros O2KFluorometer. The core will run samples, assist, train, and/or supervise data analysis from basic data reduction, incorporating procedural control, normalization, pathway ontology matching, data interpretation, and offer possible next steps. Aim 3: Perform sample processing, technical analyses, and data reduction-interpretation-presentation. The core will assist users by performing sample preparation, instrument operation, mass spectrometry peak matching to metabolites with a known compound library, sample normalization, and statistical analyses. The core will utilize integrated processes established by core leadership from experimental design though rapid and consistent sample acquisition, sample fixation, sample extraction, data reduction, statistical analysis, data analysis, and biological interpretation to ensure the success of the CNS-Met RPLs and pilot project PIs.