SURFACE ANTIGENS OF M LEPRAE AND OTHER MYCOBACTERIA

这项提案的核心是新发现的， 脂寡糖抗原，普遍存在于非典型（非结核） 结核分枝杆菌，可能存在于结核分枝杆菌和 麻风分枝杆菌 来自堪萨斯分枝杆菌的那些被表征为 通过含有糖苷海藻糖的“核心”，由物种特异性 糖和酰基取代基。 最先进的化学分析（例如360 MHz 1H-和13 C-NMR;气液色谱/质谱;快原子 轰击/质谱法）将被应用，探测各种 含海藻糖的“核心”和种特异性的关系 糖和酰基功能的“核心”。 分析的基础 含酸的“C-真菌苷”糖肽脂（GPL）抗原 鸟分枝杆菌/胞内分枝杆菌/结核分枝杆菌 复合物将继续与它们的生物合成的探测相一致， 基于它们起源于水溶性糖肽的观察 对D环丝氨酸敏感。 溶原性转化在 自然界中含有糖肽脂的血清型的多样性 被探索。 此外，种属特异性的临床应用 糖脂，纳入酶联免疫吸附测定， 病原体鉴定和疾病的直接血清诊断将 继续 生理功能和在发病机制/持续性中的作用 的物种特异性脂寡糖/糖肽脂将是 重点探讨了惰性类脂胶囊的特性。

The centerpiece of this proposal is the new found, extraordinary lipooligosaccharide antigens, ubiquitous among atypical (nontuberculous) mycobacteria and perhaps present in Mycobacterium tuberculosis and Mycobacterium leprae. Those from Mycobacterium kansasii are characterized by a glycosidic trehalose-containing "core", modulated by species-specific sugars and acyl substituents. State-of-the-art chemical analysis (e.g. 360 MHz 1H- and 13C-NMR; gas liquid chromatography/mass spectrometry; fast atom bombardment/mass spectrometry) will be applied, probing the variety of trehalose-containing "cores" and the relationship of species-specific sugars and acyl functions to the "cores". Analysis of the pyruvic acid-containing "C-mycoside" glycopeptidolipid (GPL) antigens of the Mycobacterium avium/Mycobacterium intracellulare/Mycobacterium scrofulaceum complex will continue in concert with a probing of their biosynthesis, based on observations that they originate in water-soluble glycopeptides and are susceptible to D cycloserine. The role of lysogenic conversion in the multiplicity of glycopeptidolipid-containing serotypes in nature will be explored. In addition, the clinical application of the species-specific glycolipids, incorporated into enzyme linked immunosorbent assays, in identification of pathogens and direct serodiagnosis of disease will continue. The physiological function and role in pathogenesis/persistence of the species-specific lipooligosaccharides/glycopeptidolipids will be explored with emphasis on the theorm of an inert lipoidal capsule.