ANTIGENIC/IMMUNOGENIC COMPONENTS--BORRELIA BURGDORFERI

莱姆病是一种由壁虱传播的人畜共患病。主要按蚊媒介进行了研究。 Burgdorferi是缺乏寄主专一性的硬体动物。控制 这种疾病通过减少水塘的啮齿动物数量和 更大的哺乳动物宿主是不切实际的，实际上可能是 适得其反。杀螨剂的广泛使用是值得怀疑的 由于缺乏有效性，可能会对环境造成不利影响 具有专一性。年控制莱姆病的重要一步 家畜和人类的发展是一种安全、有效和 相对便宜的疫苗。年莱姆病病例数 家畜和人类的数量正在增加。莱姆病是一种复杂的 人畜共患病维持在自然疫源地，其媒介与动物之间 主持人。休闲活动和亲近自然的生活产生了 在更大程度上暴露在扁虱媒介中。 我们已经证明，仓鼠可以通过以下方式保护自己免受实验性感染 伯氏杆菌通过被动或主动免疫。这表明 保护两种家畜的莱姆病疫苗的可行性 人类也不会感染这种疾病。金黄色葡萄球菌的鉴定与表征 负责对感染免疫的伯氏杆菌抗原(S)将 促进开发安全有效的莱姆病疫苗 疾病。此外，这些研究应该会增加我们对 对伯氏杆菌的免疫反应的性质。 这项建议的具体目标是： 1)确定不同地理区域伯氏杆菌的免疫类型， 2)鉴定伯氏杆菌的抗原组分(S) 具有免疫分型特异性；3)鉴定鸡传染性支气管炎病毒的抗原组分(S 伯氏杆菌与免疫型特异性的关系，4)调查 免疫型特异性抗体与伯氏杆菌的相互作用及5)对 开发寡核苷酸探针以确定病毒的物理位置 表达伯氏杆菌免疫原性成分(S)的基因(S)。

Lyme disease is a tick-borne zoonosis. The major tick vectors of B. burgdorferi are Ixodes species which lack host specificity. Control of this disease through reducing the population of the rodent reservoirs and larger mammalian hosts is impractical and may actually be counterproductive. The widespread use of acaricides is of questionable effectiveness and may have adverse environmental effects due to their lack of specificity. A major step toward the control of Lyme disease in domestic animals and humans is the development of a safe, effective and relatively inexpensive vaccine. The number of cases of Lyme disease in domestic animals and humans are increasing. Lyme disease is a complex zoonosis maintained in natural foci between its tick vectors and animal hosts. Recreational activities and living close to "nature" has resulted in greater exposure to the tick vectors. We have shown that hamsters can be protected from experimental infection by B. burgdorferi by either passive or active immunization. This suggests the feasibility of a Lyme disease vaccine for protecting both domestic animals and humans from this disease. The identification and characterization of the antigen(s) of B. burgdorferi responsible for immunity to infection will facilitate the development of a safe and effective vaccine for Lyme disease. In addition, these studies should increase our understanding of the nature of the immune response to B burgdorferi. The specific aims of this proposal are: 1) To identify immunotypes of B. burgdorferi in various geographical areas, 2) to identify the antigenic components(s) of B. burgdorferi associated with immunotype specificity, 3) to characterize antigenic components(s) of B. burgdorferi associated with immunotype specificity, 4) to investigate interaction of immunotype specific antibody with B. burgdorferi and 5) to develop oligonucleotide probes to identify the physical location of the gene(s) that express the immunogenic components(s) of B. burgdorferi.