MALE REPRODUCTIVE PHYSIOLOGY

这项研究的长期目标是阐明 精子发育的机制 哺乳动物附睾体的受精能力。作为精子 穿过附睾，它们就会依次暴露在 不同的附睾腺环境由分泌物和 附睾细胞的吸收。工作假说是， 精子所需的连续质膜的成熟- 鸡蛋的相互作用取决于与之的顺序相互作用 激素控制的附睾分泌蛋白。以下是 已开发出以下程序：1)体外维护 附睾外植体中所有细胞之间的相互作用 一个区域及其三维结构关系是 保存，以及2)分离和培养有活力的上皮和 肾小管周围附睾腺细胞。 这项建议的具体目标是：1)研究蛋白质 附睾糖蛋白的合成和分泌。这 在加入标记糖和氨基后，将进行研究 酸，经SDS-PAGE电泳法和免疫沉淀法；2)至 蛋白质和糖蛋白合成的激素调控研究 和分泌；3)测定分泌蛋白的结合和 附睾精子糖蛋白及其对精子的影响 精子与卵子结合，进而影响精子受精能力；4) 确定附睾分泌物的地区差异和 分泌-精子相互作用：5)研究附睾液的分泌 可以修饰精子表面的糖苷酶；6)研究 糖苷酶抑制剂诱导的细胞结构和功能改变 分泌附睾糖蛋白和精子糖蛋白；7)研究附睾 单胎小鼠的分泌及其与精子的相互作用 精子产生正常、精子形态正常的基因突变， 精子活力差，受精率低。兔子、大鼠和小鼠会 被利用。这些研究应该会为 男性生育问题。

The long term objective of this research is the elucidation of the mechanisms which are responsible for the development of sperm fertilizing capacity in the mammalian epididymis. As spermatozoa pass through the epididymis, they are sequentially exposed to varying epididymal environments created by the secretion and absorption of epididymal cells. The working hypothesis is that the maturation of the sequential plasma membrane necessary for sperm- egg interaction is dependent upon the sequential interaction with hormonally controlled epididymal secretory proteins. The following procedures have been developed: 1) maintenance in vitro of epididymal explants where the interaction between all the cells of a region and their 3-dimensional structural relationship are preserved, and 2) isolation and culture of viable epithelial and peritubular epididymal cells. The specific objectives of this proposal are 1) to study protein and glycoprotein synthesis and secretion by epididymal cells. This will be studied, after incorporation of labeled sugar and amino acids, by SDS-PAGE electrophoresis and immunoprecipitation; 2) to study the hormonal control of protein and glycoprotein synthesis and secretion; 3) to determine the binding of secreted proteins and glycoprotein to epididymal spermatozoa and their effect first on sperm-egg binding and then on sperm fertilizing ability; 4) to determine the regional differences in epididymal secretion and secretion-sperm interactions: 5) to study the epididymal secretion of glycosidases which can modify the sperm surface; 6) to study glycosidase inhibitor-induced changes in structure and function of secreted epididymal and sperm glycoproteins; 7) to study epididymal secretion and secretion-sperm interaction in mice bearing single gene mutations with normal sperm production, sperm morphology, sperm motility, and low fertility. Rabbits, rats, and mice will be used. These studies should provide important insights into the problem of male fertility.