NEURAL REGULATION OF SHALLOW DAILY TORPOR

浅表的日常麻木，就像冬眠一样，是哺乳动物的一种夸张形式 睡吧。因此，了解参与调节的神经机制 无论是浅层的麻木状态还是深度的冬眠状态都能帮助我们洞察神经。 控制负责调节正常的恒温哺乳动物睡眠和 保持清醒。因此，拟议研究计划的最终目标是 是为了了解浅层日常麻木的神经控制。具体的 有趣的问题是：1)哪些神经调节系统控制着 进入麻木和从麻木中唤醒？以及，2)其他神经如何？ 降低体温对调节机制的影响 伴随着麻木？这项建议所包括的研究项目包括 旨在帮助回答问题1，并将专门测试 肌肉紧张症的产生是一种必要的组织的假说 导致进入浅麻木状态的事件。第一个子项目 将使用去大脑动物和电微刺激来确定 脑桥和延髓松弛中心的立体定位坐标。这个 第二个子项目将使用去大脑的动物和化学微 刺激证明脑干松弛中心是 胆碱感受剂。第三个子项目将长期使用动物 植入针对脑干张力障碍的显微注射插管 化学性肌肉紧张症的中心和文献制作 微刺激。最后的项目将使用这些相同的动物来 确定微量注射药理剂量的卡巴胆碱是否 松弛中枢导致浅层麻木。此外，这项研究建议 旨在允许学生大量参与。这名学生将成为 接受过神经生理学研究中广泛使用的基本技术培训。 他/她还将学习基本的实验设计、数据简化和 统计分析，以及稿件和研讨会的准备。这些 经验将提供坚实的基础，并促进人们对更多 生物医学研究方面的高级培训。

Shallow daily torpor, like hibernation, is an exaggerated form of mammalian sleep. Thus, understanding the neural mechanisms involved in regulation of either shallow torpor or deep hibernation will lend insight into the neural controls responsible for regulating normal euthermic mammalian sleep and wakefulness. Consequently, the ultimate goal of the proposed research plan is to understand the neural control of shallow daily torpor. The specific questions of interest are: 1) Which neural regulatory systems control the entrance into and arousal from torpor?, and, 2) How are other neural regulatory mechanisms influenced by the reduced body temperature which accompanies torpor? the research projects included in this proposal are designed to help answer question #1 and will specifically test the hypothesis that production of muscular atonia is an essential organizing event which leads to entrance into shallow torpor. The first sub-project will use decerebrate animals and electrical microstimulation to determine stereotaxic coordinates for pontine and medullary atonia centers. The second sub-project will use decerebrate animals and chemical micro- stimulation to document that the brainstem atonia centers are cholinoceptive. The third sub-project will use animals chronically implanted with microinjection cannulas aimed at the brainstem atonia centers and document production of muscular atonia following chemical microstimulation. The final project will use these same animals to determine if microinjection of pharmacological doses of carbachol into the atonia centers induces shallow torpor. In addition, this research proposal is designed to allow substantial student involvement. The student will be trained in basic techniques widely utilized in neurophysiological research. He/she will also learn basic experimental design, data reduction and statistical analysis, and manuscript and seminar preparation. These experiences will provide a sound base for and promote interest in more advanced training in biomedical research.