INTERCELLULAR COMMUNICATION IN THE LENS

研究方案的长期目标是获得详细的 关于3个透镜光纤间隙连接的功能特性的信息 蛋白质和连接蛋白结构与功能的关系。 详细了解这些过程是很重要的，如果我们要 了解不同连接蛋白的功能特性如何起作用 器官动态平衡和白内障是如何发生的。具体目标有5个： 1.进一步表征3个镜头间隙的功能特性 在非洲爪哇卵母细胞对中表达的连接蛋白。 2.表征单个、非交界性cx46诱导的行为 孤立卵母细胞中的通道。 3.确定CX46和CX50的羧基末端结构域的作用 在渠道功能方面。 4.确定其他蛋白结构域在通道门控和 渗透。 5.鉴定大鼠CX46和大鼠CX50的功能特性 利用双膜片钳技术转染间隙连接蛋白的哺乳动物细胞系 技术。

The long term objectives of the research proposal are to obtain detailed information about the functional properties of 3 lens fiber gap junctional proteins and the relationship between connexin structure and function. Detailed knowledge about these processes is important if we are to understand how the functional properties of different connexins contribute to organ homeostasis and how cataracts arise. There are 5 specific aims: 1. To characterize further the functional properties of 3 lens gap junctional proteins expressed in Xenopus oocyte pairs. 2. To characterize the behavior of single, nonjunctional cx46-induced channels in solitary oocytes. 3. To determine the role of the carboxyl terminal domain of cx46 and cx50 in channel function. 4. To determine the role of other protein domains in channel gating and permeation. 5. To characterize the functional properties of rat cx46 and rat cx50 in connexin-transfected mammalian cell lines using the dual patch clamp technique.