YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROL

本研究的目的是阐明真核基因的作用机制 调控 选择的研究系统是酵母调节途径 由基因CCR 4、CRE 1和CRE 2定义。 需要CCR 4， 葡萄糖抑制性醇脱氢酶（ADH 2）和其他 非发酵基因 CRE 1和CRE 2是抑制 CCR 4或其他需要CCR 4发挥功能的因素。 CRE基因 与SPT基因家族的两个成员等位，其中突变 抑制HIS 4和LYS 2基因座的启动子插入。 CCR 4基因是 也是SPT介导的基因表达所必需的。 小组委员会的一些成员 一个基因家族被鉴定为组蛋白，另一个基因家族被鉴定为TATA结合蛋白 蛋白 CRE/SPT基因作为几种调节因子的鉴定 显然不相关的系统，作为染色质的组成部分， 转录复合物表明，这些调节因子，连同CCR 4， 在维持基因的一般转录控制中起关键作用 表情 CCR 4/CRE基因的分子作用将通过一个组合来研究。 基因、生化和重组DNA技术。 功能 CCR 4和CRE 1蛋白的区域将通过缺失来鉴定， 突变分析，并将鉴定与CCR 4结合的蛋白质 通过免疫学和生化技术。 转录后 CRE基因对CCR 4的调节将通过检测CRE基因的表达来探索。 CCR 4与CRE 1和CRE 2的潜在蛋白质-蛋白质相互作用， 确定cre突变对CCR 4蛋白丰度的影响。 的 ccr 4和cre突变对ADH 2染色质结构的影响将是 确定和网站在ADH 2通过这些因素的行动将是 鉴定 还将鉴定CCR 4功能所需的其他基因 通过传导额外的突变体狩猎，以及其他SPT的潜在作用， 将检测ADH 2调节基因。

The goal of this research is to elucidate the mechanisms of eucaryotic gene regulation. The system chosen for study is the yeast regulatory pathway defined by the genes CCR4, CRE1, and CRE2. CCR4 is required for the transcription of glucose-repressible alcohol dehydrogenase (ADH2) and other non-fermentative genes. CRE1 and CRE2 are negative effectors that repress CCR4 or some other factor that requires CCR4 for function. The CRE genes are allelic to two members of the SPT gene family, mutations in which suppress promoter insertions at the HIS4 and LYS2 loci. The CCR4 gene is also required for spt-mediated gene expression. Some members of the SPT gene family have been identified as histones and one as the TATA binding protein. The identification of the CRE/SPT genes as regulators of several apparently unrelated systems and as integral components of chromatin and transcription complexes suggests that these regulators, together with CCR4, play key roles in maintaining general transcriptional control of gene expression. The molecular role of the CCR4/CRE genes will be studied by a combination of genetic, biochemical, and recombinant DNA techniques. The functional regions of CCR4 and CRE1 proteins will be identified by deletion and mutation analysis, and the proteins which bind to CCR4 will be identified by immunological and biochemical techniques. The post-transcriptional regulation of CCR4 by the CRE genes will be explored by examining the potential protein-protein interaction of CCR4 with CRE1 and CRE2 and by determining the effect of cre mutations on CCR4 protein abundance. The effects of ccr4 and cre mutations on ADH2 chromatin structure will be determined and the site at ADH2 through which these factors act will be identified. Other genes required for CCR4 function will also be identified by conduction additional mutant hunts, and the potential role of other SPT genes in ADH2 regulation will be examined.