GENETICS AND BIOCHEMISTRY OF PLASMID TRANSFER

质粒DNA元件在细菌中的传播 提供了一种最重要的机制，通过它 细菌获得新的致病特征和其他基因 使临床治疗复杂化的特征。使用F 以大肠杆菌的质粒接合系统为模型，我们是 研究基因和基因产品所需的 F DNA从一个细胞传到另一个细胞。在我们的 F_1转移中基因的遗传和蛋白质产物分析 我们已经发现了一些以前没有被认识到的 编码序列，以及所识别的活动 制作F-皮林。我们现在计划完成DNA序列 含有转移基因跟踪的F DNA片段的分析 通过trbB，刻画了与转移相关的函数 这一地区的新基因座。我们还提出了专门设计的实验 F-毛细蛋白合成的生化步骤表征及初步研究 TRAQ和其他TRA职能在这一过程中的作用。

The dissemnation of plasmid DNA elements among bacteria provides one of the most important mechanisms through which bacteria gain new pathogenic traits and other genetic characteristics that complicate clinical therapy. Using the F plasmid conjugation system of Escherichia coli as a model, we are studying the genes and gene products required for the transmission of F DNA from one cell to another. During our genetic and protein product analysis of genes in the F transfer region we have discovered a number of previously unrecognized coding sequences, and identified activities that are involved in making F-pilin. We now plan to complete a DNA sequence analysis of the F DNA segment which includes transfer genes traC through trbB and to characterize the transfer related function of the new loci in this region. We also propose experiments designed to characterize the biochemical steps of F-pilin synthesis and the role of traQ and other tra functions in this process.