BLOOD PRESSURE CONTROL BY FOREBRAIN & BRAINSTEM NEURONS

描述：(改编自申请者的摘要)远景目标 这项研究的目的是描述控制中枢神经系统的通路 基础交感神经放电(SND)和心血管功能。 自1990年以来，PI一直在研究10赫兹节奏的控制 SND的主要特征是去大脑或氨基甲酸乙酯- 麻醉的猫。这种节奏在神经中高度连贯。 控制各种心血管靶器官，可能是 诱发脊髓交感神经复杂模式的前提条件 伴随着某些行为状态的流出。在研究过程中 目前的资助期是第一个确定特定CNS的 规范这一活动的站点。逆行激活的数据 实验表明，在某些情况下， 这些区域和药理学研究表明神经递质 在SND的10赫兹节律控制中起重要作用的系统。 在拟议的5年研究计划中，最先进的电生理学 分析技术将与微离子导入相结合 和微量注射药物，以解决4个具体目标：(1)测试 假设A5、A6和臂旁/Kolliker的脑桥神经元- 引信复合体参与10赫兹节律的控制，(2) 识别相互联系和信号(兴奋或抑制) 脑干神经元之间的突触联系与活动 与SND中的10赫兹节律相关，(3)检验假设 10赫兹节律是由脑干的分布式网络产生的 神经元，以及(4)确定5-羟色胺能和 儿茶酚胺能神经元影响10赫兹之间的平衡 SND的节律和2-6 Hz的不规则振荡。这些研究 将增进我们对负责的中央电路的了解 用于产生和控制静息SND中的10赫兹节律。

DESCRIPTION: (adapted from applicant's abstract) The long-range goal of this research is to characterize the CNS pathways that control basal sympathetic nerve discharge (SND) and cardiovascular function. Since 1990, the PI has studied the control of the 10-Hz rhythm that is the predominant feature of SND in decerebrate or urethane- anesthetized cats. This rhythm is highly coherent in nerves controlling a variety of cardiovascular target organs and may be a prerequisite for inducing the complex patterns of spinal sympathetic outflow that accompany some behavioral states. Studies during the current funding period are the first to identify specific CNS sites that regulate this activity. Data from antidromic activation experiments have indicated reciprocal connections between some of these areas and pharmacologic studies have suggested neurotransmitter systems that are important in the control of the 10 hz rhythm in SND. In the proposed 5-yr research plan, state-of-the-art electrophysiologic and analytic techniques will be combined with microiontophoresis and microinjection of drugs to address 4 Specific aims: (1) To test the hypothesis that pontine neurons in the A5, A6 and parabrachial/Kolliker- Fuse complex are involved in the control of the 10 Hz rhythm, (2) To identify the interconnections and signs (excitation or inhibition) of synaptic connections among brainstem neurons with activity correlated to the 10 Hz rhythm in SND, (3) To test the hypothesis that the 10 Hz rhythm is generated by a distributed network of brainstem neurons, and (4) to identify the central sites where serotonergic and catecholaminergic neurons act to affect the balance between the 10 Hz rhythm and the irregular 2-6 Hz oscillations in SND. These studies will enhance our understanding of the central circuitry responsible for generating and controlling the 10 Hz rhythm in resting SND.