SEX HORMONE EFFECTS ON LIPOPROTEIN SPECIATION

美国4300万名女性将于#年满50岁 而冠状动脉疾病(CAD)将是这十年的主要原因 在这群人中的死亡。因此，了解风险是当务之急。 参与冠心病病理生理学的因素。其中， 脂蛋白的改变起着主要作用。然而，还有很多事情要做。 了解各种形式的高密度脂蛋白和低密度脂蛋白 和低密度脂蛋白)及其调控。 因此，这项研究的目的是阐明 内源性和外源性雌激素、孕激素和雄激素在物种形成中的作用 育龄妇女和绝经后妇女高密度脂蛋白和低密度脂蛋白的组成。 为了实现这一目标，将对三组妇女进行研究： 1)月经周期正常的妇女[服药前后 促性腺激素释放激素(GnRH)类似物]；2)高雄激素女性 生育年龄(服用促性腺激素释放激素类似物前后 未添加雌激素]；以及3)绝经后妇女[一组 在没有外源性类固醇的情况下，一组只接受雌激素，第三组 雌激素组和孕激素组]。所有研究中的女性都将拥有 促性腺激素、性类固醇(雌激素、雄激素、黄体酮)和性 激素结合球蛋白，用放射免疫法测定。这些测量结果 将与新发现的原生生物的分布相关联 经选择性亲和免疫吸附鉴定的高密度脂蛋白的种类 免疫测定，并以低密度脂蛋白的量化形态状态进行评估 采用梯度超速离心法和电子显微镜。这些 相关性将提供对性与性之间关系的新见解 类固醇激素环境，年龄和脂蛋白，并应借给更大 了解女性冠心病的病因、治疗和预防。

Forty-three million women will reach the age of 50 in the United States in this decade, and coronary artery disease (CAD) will be the leading cause of death in this group. Therefore, it is imperative to understand the risk factors which contribute to the pathophysiology of CAD. Among these, alterations in lipoproteins play a major role. However, much remains to be learned about the various forms of high and low density lipoproteins (HDL and LDL) and their regulation. Therefore, the goal of this study is to elucidate the effects of endogenous and exogenous estrogens, progestins and androgens on speciation of HDL and LDL composition in reproductive age and postmenopausal women. To achieve this goal, studies will be performed in three groups of women: 1) women with normal menstrual cycles [before and after administration of a gonadotropin releasing hormone (GnRH) analog] ; 2) hyperandrogenic women of reproductive age (before and after administration of a GnRH analog with and without added estrogen] ; and 3) postmenopausal women [one group without exogenous steroids, one group receiving estrogen only, and a third group receiving estrogen plus progestin]. Women in all studies will have gonadotropins, sex steroids (estrogens, androgens, progesterone), and sex hormone binding globulin, measured by radioimmunoassay. These measurements will be correlated with the distribution of newly identified native species of HDL as assessed by selected affinity immunosorption and immunoassays, and with the quantized speciation state of LDL as assessed by gradient ultracentrifugation and electron microscopy. These correlations will provide new insights into the relationships between sex steroid hormone milieu, age and lipoproteins, and should lend to greater understanding of the etiology, treatment and prevention of CAD in women.