MECHANISMS OF RED NUCLEUS BURST DISCHARGE

一种新型体外制剂，利用海龟的不寻常特性 已经开发了耐缺氧性以探索 运动程序的中央代表。 这一准备工作保留了 小脑红核回路由突触连接组成 小脑、红核和网状结构之间。 阵阵 活动记录在红核在行为的猴子，猫， 海龟的运动与速度等运动参数密切相关 似乎代表了简单的运动程序。 爆发性放电也可以 在体外制剂中被诱发。 拟议的目标 实验是为了检查细胞和电路机制， 有助于在红核中产生爆发放电， 以此类推，也就是运动程序的产生。 细胞外和细胞内 红核神经元的记录结合离子置换 和活性依赖性染料的应用将被执行，以研究 周期性兴奋和内在膜的相对贡献 在突发放电的生产性能。 机制也必须 修改移动命令，使其适应 环境 可能用于调节红核运动的机制 输出可能包括神经递质或神经调质，如GABA 或者血清素 将对突发放电的精细控制进行研究， 用细胞外记录和离子电渗法检查 神经递质及其拮抗剂。 这些关于细胞的研究 和突触整合的电路基础， nucleus将提供更好地了解 小脑红核突触通路对适应性运动的作用 控制和运动障碍，特别是帕金森病。

A novel in vitro preparation that takes advantage of the turtle's unusual resistance to anoxia has been developed to explore mechanisms of the central representation of motor programs. This preparation preserves the cerebellorubral circuit which is comprised of synaptic connections between the cerebellum, red nucleus and reticular formation. Bursts of activity recorded in the red nucleus in behaving monkeys, cats and turtles correlate closely with parameters of movement such as velocity and appear to represent simple motor programs. Burst discharges can also be elicited in the in vitro preparation. The goals of the proposed experiments are to examine the cellular and circuit mechanisms that contribute to the generation of burst discharges in the red nucleus, and by analogy, to motor program generation. Extracellular and intracellular recordings of red nucleus neurons in combination with ion substitution and application of activity-dependent dye will be performed to study the relative contributions of recurrent excitation and intrinsic membrane properties in the production of burst discharges. Mechanisms must also be present to modify movement commands so that they are adaptive to the environment. Mechanisms that might be used to adjust red nucleus motor output could include neurotransmitters or neuromodulators, such as GABA or serotonin. Studies of the fine control of burst discharges will be examined with extracellular recordings and iontophoresis of neurotransmitters and their antagonists. These studies of the cellular and circuit bases of synaptic integration underlying activity in the red nucleus will provide new data required to better understand the contribution of cerebellorubral synaptic pathways to adaptive motor control and to movement disorders, particularly Parkinson's disease.