NERVE GUIDE--NEUROSURGICAL ALTERNATIVE FOR NERVE REPAIR

这项研究的长期目标是在体内测试， 微环境对小鼠轴突再生的影响 外周神经系统。 我们将继续发展解剖学和 生理学方法和技术，将定量评估 周围神经修复后的功能和解剖恢复。 具体目标I和II将量化感觉器官的再生反应， 运动神经元和它们的轴突。 这一量化将在 对于不同的实验条件，系统地改变 再生轴突的微环境。 具体目标三至五： 再生轴突与其目标的相互作用。 这些目标 实验的目的是确定有多少轴突长回合适的位置， 目标，一旦在目标中，他们中有多少人建立解剖学和 功能性的生理联系。 具体目标六将使用 定量方法与以往的具体目标直接比较 周围神经修复的各种标准程序的功效。 这些研究的结果将提供定量基线数据 关于周围神经修复后的功能恢复。 直接 功能神经的解剖学和生理学测量的相关性 再生将被用来比较不同的外周的功效 神经修复策略 这种定量比较直接 与人类外周神经修复的临床相关性。

The long term objectives of this research are to test, in vivo, the influence of the microenvironment on axonal regeneration in the mouse peripheral nervous system. We will continue to develop anatomic and physiologic methods and techniques which will quantitatively assess functional as well as anatomical recovery following peripheral nerve repair. Specific Aims I and II will quantify the regeneration response of sensory and motor neurons and their axons. This quantification will be carried out for different experimental conditions which systematically alter the microenvironment of the regenerating axons. Specific Aims III-V focus on interactions of the regenerated axons with their target. The goal of these experiments is to establish how many axons grow back to their appropriate targets, and once in the target how many of them establish anatomical and physiological connections which are functional. Specific Aim VI will use the quantitative methods of the previous specific aims to directly compare the efficacy of various standard procedures for peripheral nerve repair. The results of these studies will provide quantitative baseline data concerning functional recovery following peripheral nerve repair. Direct correlation of anatomical and physiological measures of functional nerve regeneration will be used to compare the efficacy of different peripheral nerve repair strategies. Such quantitative comparisons have direct clinical relevance to human peripheral nerve repair.